Evidence map›Paper›PMID 40413349›Full record

ArticleInflammation research : official journal of the European Histamine Research Society ... [et al.]2025

Lung contusion drives lung injury by modifying miRNA cargo in alveolar small extracellular vesicles.

Keita Nakatsutsumi, Dong Jun Park, Wooil Choi, William Johnston, Katie Pool, Jenny Kezios, Raul Coimbra, Brian P Eliceiri, Todd W Costantini

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In one paragraph

Article in Inflammation research : official journal of the European Histamine Research Society ... [et al.], 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Keita NakatsutsumiDivision of Trauma, Surgical Critical Care, Burns and Acute Care Surgery, Department of Surgery, UC San Diego School of Medicine, 200 West Arbor Drive, San Diego, CA, 92103-8896, USA.
Dong Jun ParkDivision of Trauma, Surgical Critical Care, Burns and Acute Care Surgery, Department of Surgery, UC San Diego School of Medicine, 200 West Arbor Drive, San Diego, CA, 92103-8896, USA.
Wooil ChoiDivision of Trauma, Surgical Critical Care, Burns and Acute Care Surgery, Department of Surgery, UC San Diego School of Medicine, 200 West Arbor Drive, San Diego, CA, 92103-8896, USA.
William JohnstonDivision of Trauma, Surgical Critical Care, Burns and Acute Care Surgery, Department of Surgery, UC San Diego School of Medicine, 200 West Arbor Drive, San Diego, CA, 92103-8896, USA.
Katie PoolDivision of Trauma, Surgical Critical Care, Burns and Acute Care Surgery, Department of Surgery, UC San Diego School of Medicine, 200 West Arbor Drive, San Diego, CA, 92103-8896, USA.
Jenny KeziosDivision of Trauma, Surgical Critical Care, Burns and Acute Care Surgery, Department of Surgery, UC San Diego School of Medicine, 200 West Arbor Drive, San Diego, CA, 92103-8896, USA.
Raul CoimbraComparative Effectiveness and Clinical Outcomes Research Center, Riverside University Health System, Loma Linda University School of Medicine, 26520 Cactus Ave, Moreno Valley, CA, 92555, USA.
Brian P EliceiriDivision of Trauma, Surgical Critical Care, Burns and Acute Care Surgery, Department of Surgery, UC San Diego School of Medicine, 200 West Arbor Drive, San Diego, CA, 92103-8896, USA.
Todd W CostantiniDivision of Critical Care and Acute Care Surgery, Department of Surgery, University of Minnesota Medical School, 420 Delaware St. SE, MMC 195, Minneapolis, MN, 55424, USA. cost0086@umn.edu.

Funding

Mechanism of action of uniquely human genes in the injury responseR35GM149345 · NIGMS · UNIVERSITY OF MINNESOTA · PI Todd W Costantini · 2023 to 2026
$1.5M
NIGMS NIH HHS R35 GM149345NIH HHS 5R35GM149345-02
6 · The paper itself

Abstract

objectiveTo evaluate the micro-RNA (miRNA) cargo of alveolar small extracellular vesicles (sEVs) after lung contusion (LC), which can contribute to the development of trauma-related acute lung injury (ALI).

methodsA mouse model of LC was conducted with a controlled cortical impact device. Bronchoalveolar lavage fluid (BAL) was collected 24 h post-injury and sEVs were purified using size exclusion chromatography. sEVs characteristics and miRNA cargo were analyzed with vesicle flow cytometry and sequencing. Macrophages were treated with BAL sEVs in vitro to assess their pro-inflammatory effect.

resultsLC increased lung permeability and caused ALI histologically. LC increased the number of sEVs in the BAL and altered their miRNA cargo. BAL sEVs collected after LC increased pro-inflammatory cytokine release from macrophages.

conclusionLC increased the mobilization of sEVs to the alveolar space and modified their miRNA cargo that might contribute to the development of ALI by activating the immune response in macrophages.

Indexed as

Acute Lung InjuryContusionsExtracellular VesiclesLung InjuryMicroRNAsAnimalsBronchoalveolar Lavage FluidCytokinesDisease Models, AnimalLungMacrophagesMaleMiceMice, Inbred C57BLCytokinesMicroRNAsAcute lung injuryBronchoalveolar lavage fluidExtracellular vesiclesLung contusionMicro RNATrauma

Identifiers

PMID40413349
PMCPMC12103328

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.