Evidence map›Paper›PMID 40411785›Full record

ArticleCell reports2025

MePCE promotes homologous recombination through coordinating R-loop resolution at DNA double-stranded breaks.

Sravan K Devanathan, Yi-Ru Li, Samantha B Shelton, Joshuah Nguyen, Wei-Che Tseng, Nakul M Shah, Marvin Mercado, Kyle M Miller, Blerta Xhemalçe

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Mepce Restrains Ferroptosis in Prostate Cancer Through 7SK-P-TEFb Pause Control.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026
    Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Sravan K DevanathanDepartment of Molecular Biosciences, University of Texas at Austin, 2500 Speedway, Austin, TX 78712, USA.
Yi-Ru LiDepartment of Molecular Biosciences, University of Texas at Austin, 2500 Speedway, Austin, TX 78712, USA; Department of Biochemistry and Winship Cancer Center, Emory University School of Medicine, 1510 Clifton Road NE, Atlanta, GA 30322, USA.
Samantha B SheltonDepartment of Molecular Biosciences, University of Texas at Austin, 2500 Speedway, Austin, TX 78712, USA.
Joshuah NguyenDepartment of Molecular Biosciences, University of Texas at Austin, 2500 Speedway, Austin, TX 78712, USA; Department of Biochemistry and Winship Cancer Center, Emory University School of Medicine, 1510 Clifton Road NE, Atlanta, GA 30322, USA.
Wei-Che TsengDepartment of Molecular Biosciences, University of Texas at Austin, 2500 Speedway, Austin, TX 78712, USA; Department of Radiation Oncology and Winship Cancer Center, Emory University School of Medicine, 1750 Haygood Drive NE, Atlanta, GA 30307, USA.
Nakul M ShahDepartment of Molecular Biosciences, University of Texas at Austin, 2500 Speedway, Austin, TX 78712, USA.
Marvin MercadoDepartment of Molecular Biosciences, University of Texas at Austin, 2500 Speedway, Austin, TX 78712, USA.
Kyle M MillerDepartment of Molecular Biosciences, University of Texas at Austin, 2500 Speedway, Austin, TX 78712, USA; Department of Radiation Oncology and Winship Cancer Center, Emory University School of Medicine, 1750 Haygood Drive NE, Atlanta, GA 30307, USA.
Blerta XhemalçeDepartment of Molecular Biosciences, University of Texas at Austin, 2500 Speedway, Austin, TX 78712, USA; Department of Biochemistry and Winship Cancer Center, Emory University School of Medicine, 1510 Clifton Road NE, Atlanta, GA 30322, USA. Electronic address: blerta.xhemalce@emory.edu.

Funding

Shaping DNA Damage Response Networks Via Histone H2A VariantsR01CA198279 · NCI · UNIVERSITY OF TEXAS AT AUSTIN · PI Kyle M Miller · 2016 to 2026
$3.3M
Elucidating mechanisms of microRNA pathway deregulation in human cellsR01GM127802 · NIGMS · UNIVERSITY OF TEXAS AT AUSTIN · PI XHEMALCE, BLERTA · 2018 to 2022
$1.7M
NCI NIH HHS R01 CA198279NIGMS NIH HHS R01 GM127802
6 · The paper itself

Abstract

MePCE is a multifunctional protein that regulates the positive transcription elongation factor b (P-TEFb) partitioning between the nucleosol and chromatin. MePCE's role in sequestering P-TEFb in the nucleosol via the 7SK ribonuclear protein complex (RNPc) is clear, but its functions on chromatin remain obscure. We report that chromatin-associated MePCE interacts with R-loop processing and DNA repair factors. MePCE is recruited to DNA double-stranded breaks (DSBs), and MePCE depletion impairs DSB repair by homologous recombination (HR), decreases RAD51 loading, and enhances R-loop levels at AsiSI-induced DSBs at specific genomic locations. Besides decreasing specific R-loop processing factors and chromatin remodelers, MePCE depletion increases the interaction with R-loops of the other constitutive member of the 7SK RNPc, LARP7, which is degraded by BRCA1/BARD1 upon DSB. Overall, our results uncover dynamic regulation of the 7SK RNPc at DSBs during the DSB repair process and explain the recently observed synthetic lethality of MePCE and BRCA1 deficiency.

Indexed as

DNA Breaks, Double-StrandedHomologous RecombinationR-Loop StructuresBRCA1 ProteinChromatinDNA RepairHumansRad51 RecombinaseBRCA1 ProteinChromatinRad51 Recombinase7SKBRCA1CP: Molecular biologyDNA repairDRIP-MSDRIP-seqFACThomologous recombinationLARP7MePCER-loops

Identifiers

PMID40411785
PMCPMC12261951

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.