Evidence map›Paper›PMID 40411663›Full record

ArticleApplied biochemistry and biotechnology2025

Simple and Cost-effective Fluoride Riboswitch-Based Whole-Cell Biosensor for the Determination of Fluoride in Drinking Water.

Madushani R Ariyarathna, Jayani P Nissanka, Kasun Methlal, Kasun D Abeyrathne, Madhusha Satharasinghe, P Banushan, Dilhara Manawadu, Gayan Mirihana Arachchilage, Gayathri N Silva

Abstract read
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In one paragraph

Article in Applied biochemistry and biotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Madushani R AriyarathnaDepartment of Chemistry, Faculty of Science, University of Colombo, Colombo 03, Sri Lanka.
Jayani P NissankaDepartment of Chemistry, Faculty of Science, University of Colombo, Colombo 03, Sri Lanka.
Kasun MethlalDepartment of Chemistry, Faculty of Science, University of Colombo, Colombo 03, Sri Lanka.
Kasun D AbeyrathneDepartment of Chemistry, Faculty of Science, University of Colombo, Colombo 03, Sri Lanka.
Madhusha SatharasingheDepartment of Chemistry, Faculty of Science, University of Colombo, Colombo 03, Sri Lanka.
P BanushanDepartment of Chemistry, Faculty of Science, University of Colombo, Colombo 03, Sri Lanka.
Dilhara ManawaduDepartment of Chemistry, Faculty of Science, University of Colombo, Colombo 03, Sri Lanka.
Gayan Mirihana ArachchilageHoward Hughes Medical Institute, Yale University, New Haven, CT, 06520-8103, USA. gmarachchilage@ptcbio.com.
Gayathri N SilvaDepartment of Chemistry, Faculty of Science, University of Colombo, Colombo 03, Sri Lanka.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Fluoride in drinking water poses health risks when exceeding WHO-recommended limits, necessitating accurate detection methods. This study developed a highly selective fluoride riboswitch (FRS)-based whole-cell biosensor using an E. coli mutant with a recombinant plasmid carrying the FRS fused to the lacZ reporter gene. Fluoride binding to the FRS aptamer triggers β-galactosidase expression, enabling fluoride quantification by a colorimetric assay. Using a calibration curve (R

Indexed as

Biosensing TechniquesDrinking WaterFluoridesRiboswitchEscherichia coliDrinking WaterFluoridesRiboswitchFluoride determinationFluoride responsive riboswitchWater qualityWhole-cell biosensor

Identifiers

PMID40411663

What OpenQuestion holds

Textmetadata
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.