Evidence map›Paper›PMID 40411288›Full record

ArticleBiotechnology and bioengineering2025

Balancing Doses of EL222 and Light Improves Optogenetic Induction of Protein Production in Komagataella phaffii.

Shannon M Hoffman, Sebastián Espinel-Ríos, Sarah K Kwartler, Makoto A Lalwani, José L Avalos

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biotechnology and bioengineering, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Structural Basis of a Novel Heme Binding Bacterial One-Component Switch.bioRxiv : the preprint server for biology · 2026
    Article
  2. Reinforcement Learning for Robust Dynamic Metabolic Control.Biotechnology and bioengineering · 2026
    Article
  3. Article
  4. Article
  5. Review
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Shannon M HoffmanDepartment of Chemical and Biological Engineering, Hoyt Laboratory, Princeton University, Princeton, New Jersey, USA.
Sebastián Espinel-RíosDepartment of Chemical and Biological Engineering, Hoyt Laboratory, Princeton University, Princeton, New Jersey, USA.
Sarah K KwartlerDepartment of Molecular Biology, Princeton University, Princeton, New Jersey, USA.
Makoto A LalwaniDepartment of Chemical and Biological Engineering, Hoyt Laboratory, Princeton University, Princeton, New Jersey, USA.
José L AvalosDepartment of Chemical and Biological Engineering, Hoyt Laboratory, Princeton University, Princeton, New Jersey, USA.ORCID https://orcid.org/0000-0002-7209-4208

Funding

This study was supported by the U.S Department of Energy, Office of Science, Office of Biological and Environmental Research Award Number DE-SC0022155, and NSF Award MCB-2300239 (to J.L.A.), as well as the Maeder Graduate Fellowship in Energy and the Environment (to S.M.H.), and NSF grant number DGE-2039656 (to S.K.K.).
6 · The paper itself

Abstract

Komagataella phaffii, also known as Pichia pastoris, is a powerful host for recombinant protein production, in part due to its exceptionally strong and tightly controlled P

Indexed as

Fungal ProteinsOptogeneticsRecombinant ProteinsSaccharomycetalesTranscription FactorsLightPromoter Regions, GeneticFungal ProteinsRecombinant ProteinsTranscription FactorsbioprocessEL222Komagataella phaffiilightoptogeneticsPichia pastorisrecombinant proteins

Identifiers

PMID40411288
PMCPMC12322658

What OpenQuestion holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.