Evidence map›Paper›PMID 40410945›Full record

ArticleMicrobial biotechnology2025

Production of Vanillin From Ferulic Acid by Pseudomonas putida KT2440 Using Metabolic Engineering and In Situ Product Recovery.

Ilona A Ruhl, Sean P Woodworth, Stefan J Haugen, Hannah M Alt, Gregg T Beckham, Christopher W Johnson

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Microbial biotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. A distinct subpopulation of membrane vesicles inApplied and environmental microbiology · 2025
    Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Ilona A RuhlRenewable Resources and Enabling Sciences Center, National Renewable Energy Laboratory, Golden, Colorado, USA.ORCID https://orcid.org/0000-0001-5478-2261
Sean P WoodworthRenewable Resources and Enabling Sciences Center, National Renewable Energy Laboratory, Golden, Colorado, USA.ORCID https://orcid.org/0000-0003-3792-9553
Stefan J HaugenRenewable Resources and Enabling Sciences Center, National Renewable Energy Laboratory, Golden, Colorado, USA.ORCID https://orcid.org/0000-0003-3999-0796
Hannah M AltRenewable Resources and Enabling Sciences Center, National Renewable Energy Laboratory, Golden, Colorado, USA.ORCID https://orcid.org/0000-0002-6559-7800
Gregg T BeckhamRenewable Resources and Enabling Sciences Center, National Renewable Energy Laboratory, Golden, Colorado, USA.ORCID https://orcid.org/0000-0002-3480-212X
Christopher W JohnsonRenewable Resources and Enabling Sciences Center, National Renewable Energy Laboratory, Golden, Colorado, USA.ORCID https://orcid.org/0000-0002-2979-4751

Funding

Bioenergy Technologies Office
6 · The paper itself

Abstract

Vanillin is the most in-demand flavouring compound in the world and because vanillin extracted from vanilla pods cannot meet the global demand, most vanillin on the market today is chemically synthesised. Increasing demands by consumers for natural ingredients have inspired efforts to develop vanillin derived from microbial sources. These efforts have been challenged by low titers, likely caused by the toxicity of vanillin to most microbial biocatalysts. In this study, we engineered a Pseudomonas putida KT2440-derived strain that accumulated vanillin from ferulic acid to 0.64 g/L. To increase the overall titre, we applied a hydrophobic polystyrene-based resin to vanillin-accumulating cultures, which enabled an increase in total vanillin recovery to an apparent titre of 3.35 g/L. This study demonstrates that P. putida can accumulate vanillin from ferulic acid to higher titers when vanillin is removed from the cultivation medium, mitigating its toxicity.

Indexed as

BenzaldehydesCoumaric AcidsMetabolic EngineeringPseudomonas putidaCulture MediaBenzaldehydesCoumaric AcidsCulture Mediaferulic acidvanillinbioconversionferulic acidin situ product recoverymetabolic engineeringPseudomonas putida KT2440vanillin

Identifiers

PMID40410945
PMCPMC12102074

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.