Evidence map›Paper›PMID 40410847›Full record

ArticleJournal of neuroinflammation2025

Gut microbiota deficiency reduces neutrophil activation and is protective after ischemic stroke.

Ali A Tuz, Susmita Ghosh, Laura Karsch, Medina Antler, Vivian Lakovic, Sabrina Lohmann, Amber Hope Lehmann, Alexander Beer, Dennis Nagel, Marcel Jung and 18 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of neuroinflammation, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Review
  2. Review
  3. Review
  4. Article
  5. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

28 authors.

Ali A Tuz *Institute for Experimental Immunology and Imaging, University Hospital Essen, University of Duisburg-Essen, 45147, Essen, Germany.
Susmita Ghosh *Leibniz-Institut Für Analytische Wissenschaften - ISAS - E.V., 44139, Dortmund, Germany.
Laura Karsch *Institute for Experimental Immunology and Imaging, University Hospital Essen, University of Duisburg-Essen, 45147, Essen, Germany.
Medina AntlerInstitute for Experimental Immunology and Imaging, University Hospital Essen, University of Duisburg-Essen, 45147, Essen, Germany.
Vivian LakovicInstitute for Experimental Immunology and Imaging, University Hospital Essen, University of Duisburg-Essen, 45147, Essen, Germany.
Sabrina LohmannInstitute for Experimental Immunology and Imaging, University Hospital Essen, University of Duisburg-Essen, 45147, Essen, Germany.
Amber Hope LehmannInstitute for Experimental Immunology and Imaging, University Hospital Essen, University of Duisburg-Essen, 45147, Essen, Germany.
Alexander BeerInstitute for Experimental Immunology and Imaging, University Hospital Essen, University of Duisburg-Essen, 45147, Essen, Germany.
Dennis NagelInstitute for Experimental Immunology and Imaging, University Hospital Essen, University of Duisburg-Essen, 45147, Essen, Germany.
Marcel JungInstitute for Experimental Immunology and Imaging, University Hospital Essen, University of Duisburg-Essen, 45147, Essen, Germany.
Nils HörenbaumInstitute for Experimental Immunology and Imaging, University Hospital Essen, University of Duisburg-Essen, 45147, Essen, Germany.
Viola KaygusuzInstitute for Experimental Immunology and Imaging, University Hospital Essen, University of Duisburg-Essen, 45147, Essen, Germany.
Altea QefaliaInstitute for Experimental Immunology and Imaging, University Hospital Essen, University of Duisburg-Essen, 45147, Essen, Germany.
Belal AlshaarLeibniz-Institut Für Analytische Wissenschaften - ISAS - E.V., 44139, Dortmund, Germany.
Niloufar AmookazemiInstitute for Experimental Immunology and Imaging, University Hospital Essen, University of Duisburg-Essen, 45147, Essen, Germany.
Silvia BolsegaInstitute for Laboratory Animal Science and Central Animal Facility, Hannover Medical School, 30625, Hannover, Lower-Saxony, Germany.
Marijana BasicInstitute for Laboratory Animal Science and Central Animal Facility, Hannover Medical School, 30625, Hannover, Lower-Saxony, Germany.
Jens T SivekeDivision of Solid Tumor Translational Oncology, German Cancer Consortium (DKTK, Partner Site Essen), German Cancer Research Center (DKFZ), Heidelberg, Germany.
Sven HeilesLeibniz-Institut Für Analytische Wissenschaften - ISAS - E.V., 44139, Dortmund, Germany.
Anika GrüneboomLeibniz-Institut Für Analytische Wissenschaften - ISAS - E.V., 44139, Dortmund, Germany.
Smiths LueongDivision of Solid Tumor Translational Oncology, German Cancer Consortium (DKTK, Partner Site Essen), German Cancer Research Center (DKFZ), Heidelberg, Germany.
Josephine HerzDepartment of Pediatrics I, Neonatology and Experimental Perinatal Neurosciences, and Center for Translational Neuro- and Behavioral Sciences (C-TNBS), University Hospital Essen, University of Duisburg-Essen, Essen, Germany.
Albert SickmannLeibniz-Institut Für Analytische Wissenschaften - ISAS - E.V., 44139, Dortmund, Germany.
Nina HagemannDepartment of Neurology, University Hospital Essen, University of Duisburg-Essen, 45147, Essen, Germany.
Anja HasenbergInstitute for Experimental Immunology and Imaging, University Hospital Essen, University of Duisburg-Essen, 45147, Essen, Germany.
Dirk M HermannDepartment of Neurology, University Hospital Essen, University of Duisburg-Essen, 45147, Essen, Germany.
Matthias GunzerInstitute for Experimental Immunology and Imaging, University Hospital Essen, University of Duisburg-Essen, 45147, Essen, Germany. matthias.gunzer@uni-due.de.
Vikramjeet SinghInstitute for Experimental Immunology and Imaging, University Hospital Essen, University of Duisburg-Essen, 45147, Essen, Germany. vikramjeet.singh@uni-due.de.

Funding

Deutsche Forschungsgemeinschaft 389030878, 405358801/428817542Deutsche Forschungsgemeinschaft 395357507Deutsche Forschungsgemeinschaft 444117227
6 · The paper itself

Abstract

Neutrophils are readily activated immune cells after ischemic stroke in mice and patients. Still, the impact of gut microbiota on neutrophil activation and its influence on inflammatory brain injury remain undefined. We report that natural microbiota colonization of germ-free (GF) mice induces substantial neutrophil activation and deteriorates stroke pathology. The colonized Ex-GF stroke mice had considerably larger infarct sizes and higher sensorimotor deficits than GF littermates. Furthermore, employing an antibiotic-based mouse model of microbiota deficiency, we demonstrate that gut microbiota depletion induces a juvenile neutrophil phenotype characterized by the upregulation of resting state surface receptors, reduced inflammatory proteins, and levels of circulating NETs. This disarming of neutrophil responses was associated with decreased expression of brain inflammatory genes, vascular thrombus formation, reduced infarct size, and alleviated behavioral deficits. We conclude that gut microbes strongly influence neutrophil activation after stroke and thus directly contribute to stroke severity.

Indexed as

Gastrointestinal MicrobiomeIschemic StrokeNeutrophil ActivationNeutrophilsAnimalsGerm-Free LifeMaleMiceMice, Inbred C57BLGut microbiotaInflammationIschemic strokeNeutrophils

Identifiers

PMID40410847
PMCPMC12100894

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.