Evidence map›Paper›PMID 40410662›Full record

ArticleBMC plant biology2025

Development and application of sex-specific indel markers for Hippophae salicifolia based on third-generation sequencing.

Zhefei Zeng, Shutong Zhang, Xin Tan, Norzin Tso, Zhuo Shang, Jifeng Zhang, Wei Li, Junwei Wang, Wenju Zhang, La Qiong

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC plant biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Nanopore Data-Driven Near-T2T Genome Assembly ofPlants (Basel, Switzerland) · 2026
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Zhefei ZengKey Laboratory of Biodiversity and Environment on the Qinghai-Tibetan Plateau, School of Ecology and Environment, Ministry of Education, Tibet University, Lhasa, China.
Shutong ZhangKey Laboratory of Biodiversity and Environment on the Qinghai-Tibetan Plateau, School of Ecology and Environment, Ministry of Education, Tibet University, Lhasa, China.
Xin TanKey Laboratory of Biodiversity and Environment on the Qinghai-Tibetan Plateau, School of Ecology and Environment, Ministry of Education, Tibet University, Lhasa, China.
Norzin TsoKey Laboratory of Biodiversity and Environment on the Qinghai-Tibetan Plateau, School of Ecology and Environment, Ministry of Education, Tibet University, Lhasa, China.
Zhuo ShangKey Laboratory of Biodiversity and Environment on the Qinghai-Tibetan Plateau, School of Ecology and Environment, Ministry of Education, Tibet University, Lhasa, China.
Jifeng ZhangKey Laboratory of Biodiversity and Environment on the Qinghai-Tibetan Plateau, School of Ecology and Environment, Ministry of Education, Tibet University, Lhasa, China.
Wei LiKey Laboratory of Biodiversity and Environment on the Qinghai-Tibetan Plateau, School of Ecology and Environment, Ministry of Education, Tibet University, Lhasa, China.
Junwei WangKey Laboratory of Biodiversity and Environment on the Qinghai-Tibetan Plateau, School of Ecology and Environment, Ministry of Education, Tibet University, Lhasa, China. jwyx12240315@126.com.
Wenju ZhangMinistry of Education Key Laboratory of Biodiversity Sciences and Ecological Engineering, State Key Laboratory of Genetic Engineering, Institute of Biodiversity Sciences and Institute of Plant Biology, School of Life Sciences, Fudan University, Shanghai, China. wjzhang@fudan.edu.cn.
La QiongKey Laboratory of Biodiversity and Environment on the Qinghai-Tibetan Plateau, School of Ecology and Environment, Ministry of Education, Tibet University, Lhasa, China. lhagchong@163.com.

Funding

Autonomous Region Innovation Training Program for College Students of Tibet University S202510694053National Innovation Training Program for College Students of Tibet University 202510694035Research and Application of Key Technologies for Biosafety and Biodiversity Conservation and Utilization in Tibet XZ202402ZD0005Science and Technology Program of Xizang Autonomous Region XZ202402ZY0023, XZ202402JX0003
6 · The paper itself

Abstract

Hippophae salicifolia, a dioecious shrub endemic to the Himalayan region, holds substantial ecological and economic value. The lack of pre-flowering morphological traits for sex identification has long impeded efficient germplasm management and breeding efforts. In this study, we utilized third-generation sequencing technology to conduct whole-genome comparative analysis of known-sex individuals, identifying insertion/deletion loci significantly associated with sex. Based on these loci, we designed a specific primer pair, Hsa09. PCR amplification results demonstrated 100% accuracy in sex differentiation, with female individuals showing a single band and male individuals exhibiting a double-band pattern. Cross-species validation revealed limited applicability of Hsa09 in closely related Hippophae species, suggesting notable divergence in sex determination mechanisms within the genus. This study establishes the first molecular tool for early sex identification in H. salicifolia, overcoming the dependence on reproductive organ development in traditional methods. It provides essential technical support for understanding the evolutionary pathways of sex chromosomes and advancing sex-regulated breeding strategies within the Hippophae genus.

Indexed as

HippophaeINDEL MutationGenetic MarkersGenome, PlantHigh-Throughput Nucleotide SequencingGenetic MarkersHippophae salicifoliaMolecular markersSex chromosomesSex determination

Identifiers

PMID40410662
PMCPMC12100851

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.