Evidence map›Paper›PMID 40410160›Full record

ArticleScientific data2025

Chromosomal scale assembly and functional annotation of the apicomplexan parasite Eimeria acervulina.

Subodh K Srivastava, Carolyn C Parker, Peter C Thompson, Matthew S Tucker, Benjamin M Rosenthal, Asis Khan, Matthew J Valente, Mark C Jenkins

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PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific data, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Subodh K SrivastavaUSDA ARS, Animal Parasitic Diseases Laboratory, Beltsville Agricultural Research Center, East, Building 1040, 10300 Baltimore Ave., Beltsville, MD, 20705, USA. Subodh.k.Srivastava@usda.gov.ORCID http://orcid.org/0000-0003-0845-0199
Carolyn C ParkerUSDA ARS, Animal Parasitic Diseases Laboratory, Beltsville Agricultural Research Center, East, Building 1040, 10300 Baltimore Ave., Beltsville, MD, 20705, USA.
Peter C ThompsonUSDA ARS, Animal Parasitic Diseases Laboratory, Beltsville Agricultural Research Center, East, Building 1040, 10300 Baltimore Ave., Beltsville, MD, 20705, USA.
Matthew S TuckerUSDA ARS, Animal Parasitic Diseases Laboratory, Beltsville Agricultural Research Center, East, Building 1040, 10300 Baltimore Ave., Beltsville, MD, 20705, USA.
Benjamin M RosenthalUSDA ARS, Animal Parasitic Diseases Laboratory, Beltsville Agricultural Research Center, East, Building 1040, 10300 Baltimore Ave., Beltsville, MD, 20705, USA.
Asis KhanUSDA ARS, Animal Parasitic Diseases Laboratory, Beltsville Agricultural Research Center, East, Building 1040, 10300 Baltimore Ave., Beltsville, MD, 20705, USA.
Matthew J ValenteUSDA ARS, Animal Parasitic Diseases Laboratory, Beltsville Agricultural Research Center, East, Building 1040, 10300 Baltimore Ave., Beltsville, MD, 20705, USA.
Mark C JenkinsUSDA ARS, Animal Parasitic Diseases Laboratory, Beltsville Agricultural Research Center, East, Building 1040, 10300 Baltimore Ave., Beltsville, MD, 20705, USA. mark.jenkins@usda.gov.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Apicomplexan parasites are single-celled obligate intracellular eukaryotic organisms that cause significant animal and human disease and pose a substantial health and socioeconomic burden worldwide. Eimeria acervulina is one such parasite of chickens, representative of several Eimeria species causing coccidiosis disease. A complete assembly of the E. acervulina genome may help identify markers of drug-resistance and design recombinant vaccines. We sequenced E. acervulina APU1 strain using Oxford Nanopore Sequencing and Illumina technology in combination with a Hi-C (Omni-C) proximity linkage library and achieved a chromosomal scale assembly using the MaSuRCA assembler. The final assembly was 52 Mb. with 15 chromosomes and 99% BUSCO completeness. A total of 7,621 genes were predicted using a pipeline of BRAKER3, GeneMark-ETP and AUGUSTUS, of which 4,647 (60.97%) have a predicted Pfam function and 1,962 (25.74%) have Gene Ontology (GO) terms matching molecular, biological, and functional classes. Stage-specific transcriptome analysis revealed 9,761 transcripts. This genome assembly and transcriptome analysis provides the foundation for identifying biologically important candidates for anticoccidial drug and vaccine development.

Indexed as

EimeriaGenome, ProtozoanAnimalsChickensChromosomesCoccidiosisMolecular Sequence Annotation

Identifiers

PMID40410160
PMCPMC12102361

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.