Evidence map›Paper›PMID 40407345›Full record

ArticleJournal of virology2025

Reconstitution of interferon regulatory factor 7 expression restores interferon beta induction in Huh7 cells.

Andreas Betz, Hao-En Huang, Zuguang Gu, Ombretta Colasanti, Teng-Feng Li, Jasper Hesebeck-Brinckmann, Nadine Gillich, Gnimah Eva Gnouamozi, Matthias Schlesner, Florian W R Vondran and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of virology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Andreas Betz *Department of Infectious Diseases, Molecular Virology, Section Virus-host interactions, Heidelberg University, Heidelberg, Germany.ORCID 0009-0002-0518-0453
Hao-En Huang *Department of Infectious Diseases, Molecular Virology, Section Virus-host interactions, Heidelberg University, Heidelberg, Germany.ORCID 0009-0005-4196-285X
Zuguang GuComputational Oncology, Molecular Diagnostics Program, National Center for Tumor Diseases (NCT) and German Cancer Research Center (DKFZ), Heidelberg, Germany.
Ombretta ColasantiDepartment of Infectious Diseases, Molecular Virology, Section Virus-host interactions, Heidelberg University, Heidelberg, Germany.
Teng-Feng LiDepartment of Infectious Diseases, Molecular Virology, Section Virus-host interactions, Heidelberg University, Heidelberg, Germany.
Jasper Hesebeck-BrinckmannDepartment of Infectious Diseases, Molecular Virology, Section Virus-host interactions, Heidelberg University, Heidelberg, Germany.
Nadine GillichDivision of Virus-Associated Carcinogenesis, German Cancer Research Center (DKFZ), Heidelberg, Germany.
Gnimah Eva GnouamoziDepartment of Infectious Diseases, Molecular Virology, Heidelberg University, Heidelberg, Germany.
Matthias SchlesnerBioinformatics and Omics Data Analysis, German Cancer Research Center (DKFZ), Heidelberg, Germany.
Florian W R VondranDepartment of General, Visceral and Transplant Surgery, Hannover Medical School, Hanover, Germany.
Stephan UrbanDepartment of Infectious Diseases, Molecular Virology, Heidelberg University, Heidelberg, Germany.
Ralf BartenschlagerDivision of Virus-Associated Carcinogenesis, German Cancer Research Center (DKFZ), Heidelberg, Germany.ORCID 0000-0001-5601-9307
Marco BinderResearch Group "Dynamics of Early Viral Infection and the Innate Antiviral Response," Division Virus-Associated Carcinogenesis, German Cancer Research Center (DKFZ), Heidelberg, Germany.ORCID 0000-0002-5805-6109
Volker LohmannDepartment of Infectious Diseases, Molecular Virology, Section Virus-host interactions, Heidelberg University, Heidelberg, Germany.ORCID 0000-0001-8719-7608

Funding

German Research Council (DFG) 272983813
6 · The paper itself

Abstract

Abundance of essential components of double-stranded RNA (dsRNA) recognition and the subsequent interferon (IFN) response vary widely between primary human hepatocytes (PHHs) and commonly used cell culture models based on derivatives of Huh7 cells, supporting replication of all hepatitis viruses. We used RNA sequencing to compare the innate immune response in hepatoma cells with primary cells and non-neoplastic immortalized hepatocytes (PH5CH). Stimulation with the dsRNA analog poly(I:C) in Huh7 and Huh7.5, either by supernatant feeding or by transfection, resulted in an induction of interferon-stimulated genes widely comparable to that of PHH and PH5CH, but Huh7 and Huh7.5 lacked efficient production of IFN-β. We identified interferon regulatory factor 7 (IRF7) as a critical component missing in Huh7-derived cells. Upon reconstitution of IRF7 expression,

Indexed as

HepatocytesInterferon-betaInterferon Regulatory Factor-7Cell Line, TumorHepacivirusHumansImmunity, InnateInterferon Regulatory Factor-3Poly I-CRNA, Double-StrandedInterferon-betaInterferon Regulatory Factor-3Interferon Regulatory Factor-7IRF3 protein, humanIRF7 protein, humanPoly I-CRNA, Double-StrandedHuh7INFBinterferonIRF3IRF7

Identifiers

PMID40407345
PMCPMC12172482

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.