ArticlemBio2025
Programming virulent bacteriophages by developing a multiplex genome engineering method.
Article in mBio, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
8 citing papers in PubMed.
- From Cloning Vectors to Phage Display: Engineering Principles and Translational Applications of Bacteriophage Display Platforms.International journal of molecular sciences · 2026Review
- Phage host range: determinants, dynamics and applications.Nature reviews. Microbiology · 2026Review
- Bacteriophage-Based Therapeutic, Diagnostic, and Biocontrol Platforms: Engineering, Evidence, and Translational Challenges.International journal of molecular sciences · 2026Review
- Defect-complementation homologous recombination: A novel strategy for precise genome engineering of virulent phages.Synthetic and systems biotechnology · 2026Article
- A fully synthetic Golden Gate assembly system for engineering aProceedings of the National Academy of Sciences of the United States of America · 2026Article
- The Role of Genomics in Advancing and Standardising Bacteriophage Therapy.Antibiotics (Basel, Switzerland) · 2026Review
- Application of bacteriophages in the prevention and control of bacterial infectious diseases in animals.Frontiers in microbiology · 2026Review
- Recent perspectives on precision-targeting therapy against oral biofilm.Journal of oral microbiology · 2026Review
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
The use of virulent bacteriophages (phages) against pathogenic bacteria has recently attracted considerable interest. The limitations of naturally isolated phages have promoted the development of genome engineering methods to optimize their functions; however, engineering of virulent phage genomes in bacterial hosts remains challenging. Here, we describe a SMART ( IMPORTANCE: Unlike temperate phages, which integrate into host genomes and allow time for genetic manipulation, lytic phages rapidly hijack the bacterial machinery and trigger host lysis within minutes, leaving an extremely narrow editing window. Furthermore, virulent phage genes usually encode toxic products that inhibit the growth of bacterial hosts. We developed a SMART (splitting, modifying, assembling, and rebooting) method for multiplex genome engineering of virulent phages. We deleted 3.9 kb sequences distributed across 8 sites in the 39.9 kb genome of the wild-type T7
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.