ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2025
Engineering Electron Transfer Flux between Cytochrome P450 Enzyme and P450 Reductase to Enhance Serotonin Production in Escherichia Coli.
Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- A second-sphere variant of cytochrome P450 PbdA improves turnover and apparent coupling in veratrate oxidation.Bioresources and bioprocessing · 2026Article
- Asymmetric Connectivity Between Redox-Active Tyrosines and Reaction-Center Chlorophylls in Photosystem II.Plants (Basel, Switzerland) · 2026Article
- Spatially harnessing oxygenase enables paired and ultraselective electrooxidative waste depolymerization.Nature communications · 2026Article
- Article
- Synergistic Tuning of Conformational Dynamics, Electron Tunneling, and Substrate Positioning Enhances Electron Transfer in a P450 Chimera for Calcifediol Biosynthesis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Engineered strains as living factories: efficient biosynthesis and modification of natural products via synthetic biology and one-pot chemical tandem reactions.Frontiers in microbiology · 2026Review
- Engineering Electron Transfer Flux between Cytochrome P450 Enzyme and P450 Reductase to Enhance Serotonin Production in Escherichia Coli.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Microbial cell factories produce valuable compounds by exploiting cytochrome P450 catalytic systems. However, the inefficient electron transfer flux (ETF) between P450 and cytochrome P450 reductase (CPR) hinders the efficient synthesis of natural products. Herein, an ETF is systematically engineered by regulating the electron transfer rate, electron-receiving rate, and electron donor NADPH availability for serotonin production. First, a putative electron transfer pathway (ETP) is identified using virtual computing and evolved based on a genetically encoded serotonin RNA biosensor. Subsequently, an intermediate site strategy is developed to shorten the electron-hopping steps and distance in the ETP of CPR for enhancing the electron transfer rate. Next, the heme-binding domain is engineered to reduce the distance between heme-Fe and the substrate channel terminal in T5H for improving the electron-receiving rate. Furthermore, the NADPH pool is enlarged to increase the electron supply for efficient catalysis of P450 systems. Finally, tryptophan-5-hydroxylase (T5H) activity (K
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Registered trials
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