Evidence map›Paper›PMID 40407180›Full record

ArticleThe Journal of organic chemistry2025

Time-Reduced N-Methylation: Exploring a Faster Synthesis Technique.

Aleksandra Helbik-Maciejewska, Agata Gitlin-Domagalska, Mladena Glavaš, Natalia Ptaszyńska, Dawid Dębowski, Anna Łęgowska, Krzysztof Rolka

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of organic chemistry, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Aleksandra Helbik-MaciejewskaDepartment of Molecular Biochemistry, Faculty of Chemistry, University of Gdansk, Wita Stwosza 63, 80-308 Gdańsk, Poland.
Agata Gitlin-DomagalskaDepartment of Molecular Biochemistry, Faculty of Chemistry, University of Gdansk, Wita Stwosza 63, 80-308 Gdańsk, Poland.ORCID 0000-0002-2583-7558
Mladena GlavašDepartment of Organic Chemistry and Biochemistry, Rud̵er Bošković Institute, Bijenička c. 54, 10 000 Zagreb, Croatia.
Natalia PtaszyńskaDepartment of Molecular Biochemistry, Faculty of Chemistry, University of Gdansk, Wita Stwosza 63, 80-308 Gdańsk, Poland.ORCID 0000-0001-7576-0248
Dawid DębowskiDepartment of Molecular Biochemistry, Faculty of Chemistry, University of Gdansk, Wita Stwosza 63, 80-308 Gdańsk, Poland.ORCID 0000-0002-5868-3276
Anna ŁęgowskaDepartment of Molecular Biochemistry, Faculty of Chemistry, University of Gdansk, Wita Stwosza 63, 80-308 Gdańsk, Poland.
Krzysztof RolkaDepartment of Molecular Biochemistry, Faculty of Chemistry, University of Gdansk, Wita Stwosza 63, 80-308 Gdańsk, Poland.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Backbone N-methylation is a pivotal peptide modification that enhances lipophilicity, metabolic stability, and binding affinity or specificity, thereby improving bioactive peptides' bioavailability. Substitution of a backbone amide hydrogen with a methyl group is a three-step procedure which is fully integrated with solid-phase peptide synthesis strategy and usually takes about 4 h. We have revolutionized this process by optimizing the method and slashing the total N-methylation procedure time from 4 h to just 40 min. Moreover, we demonstrate that N-methylation can be equally efficient regardless of the laboratory equipment used, such as a standard laboratory shaker, microwave synthesizer, or common ultrasonic bath. Our study not only results in acceleration of the N-methylation process during solid-phase peptide synthesis but also offers a flexible choice of laboratory equipment, making peptide modifications more efficient and achievable.

Indexed as

PeptidesSolid-Phase Synthesis TechniquesMethylationTime FactorsPeptides

Identifiers

PMID40407180
PMCPMC12150311

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.