ArticleJournal of photochemistry and photobiology. A, Chemistry2023
Ultrafast fluorescence dynamics of NADH in aprotic solvents: Quasi-static self-quenching unmasked.
Article in Journal of photochemistry and photobiology. A, Chemistry, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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Who cites it
2 citing papers in PubMed.
- gDAIS: A Global Analysis Application for Obtaining Confidence in FLIM.Proceedings of SPIE--the International Society for Optical Engineering · 2026Article
- Sub-100-fs energy transfer in coenzyme NADH is a coherent process assisted by a charge-transfer state.Nature communications · 2024Article
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Authors and funding
6 authors.
Funding
Abstract
We have recently provided some experimental evidence that there are ultrafast quenched conformation(s) ("dark states") of NADH. In this paper, the ultrafast fluorescence dynamics of NADH free in aprotic solvents (DMSO/DMF) has been investigated, using both a femtosecond up-conversion spectrophotofluorometer and a picosecond time-correlated single-photon counting (TCSPC) apparatus. The fluorescence kinetics in three solvents were recovered by constructing DAS (decay associated spectra) and TRES (time-resolved emission spectra). With the decrease of the ability of solvent to provide hydrogen bond interaction, apparent slow solvent relaxation (SSR) amplitudes were reduced, and the signal of "pure" quasi-static self-quenching (QSSQ) emerges. This fully positive DAS (positive at all emission wavelengths) appeared only in DMF, and this component accounts for about 20% of the total DAS. This confirms a model in which the ultrafast quenching portion of DAS is masked at times by the negative-going signal from slow solvent relaxation (SSR). Further, we have shown that the ratio of any slow solvent relaxation and QSSQ terms should be accounted for when quantifying NADH via fluorescence lifetime imaging microscopy (FLIM). Eventually, the other properties of the solvent (not only hydrogen bonding, but also polarity, viscosity, etc.) incorporated in QM-MM simulation must be fully considered to predict this ultrafast quenching vs. SSR mixture more accurately. For now, we exploit the greatly reduced H-bonding to decrypt the QSSQ in a mixture.
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