ArticleJournal of translational medicine2025
SRSF1-mediated alternative splicing regulates bladder cancer progression and cisplatin sensitivity through HIF1A/BNIP3/mitophagy axis.
Article in Journal of translational medicine, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- RRM1 competes with NEDD4 to stabilize USP19 by blocking K387 ubiquitination and suppresses autophagy-mediated chemoresistance in colorectal cancer.Cellular oncology (Dordrecht, Netherlands) · 2026Article
- NCBP2 Regulates PGAM5-Mediated Mitophagy Via KIF23 Alternative Splicing To Promote Cervical Cancer ProgressionRun Title: NCBP2 Promotes Cervical Cancer Via Mitophagy.Applied biochemistry and biotechnology · 2026Article
- Article
- Mitophagy in bladder cancer: a double-edged sword in tumor progression and therapy.Journal of translational medicine · 2026Review
- From cell lines to the clinic: identifying a urinary BlCa-EV signature through comparative proteomics of bladder cancer lysates and extracellular vesicles.Cell communication and signaling : CCS · 2026Article
- Prognostic alternative splicing signatures in esophageal cancer reveal SF3A3 as a key oncogenic splicing factor.Translational cancer research · 2026Article
- [Expression, Prognostic and Functional Analysis of SRSF Family Proteins in Non-small Cell Lung Cancer].Zhongguo fei ai za zhi = Chinese journal of lung cancer · 2026Article
- Review
- Multidimensional regulatory roles and therapeutic applications of SRSF7 in cancer.Cell death discovery · 2025Review
- Histone deacetylase HDAC7 restricts CD8 + T cell tumor infiltration and limits immunotherapy sensitivity in bladder cancer: reversal by pinocembrin.Journal of experimental & clinical cancer research : CR · 2025Article
- Letter to editor: SRSF1-mediated alternative splicing regulates bladder cancer progression and cisplatin sensitivity through HIF1A/BNIP3/mitophagy axis.Journal of translational medicine · 2025Article
- Dysregulation of mRNA splicing in urothelial carcinoma: Clinical, diagnostic and therapeutic implications.Bladder cancer (Amsterdam, Netherlands)Review
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Authors and funding
13 authors.
Funding
Abstract
backgroundAlternative splicing (AS) is consistently linked to tumor progression. SRSF1, the first identified proto-oncogene in the serine/arginine-rich splicing factor (SRSF) protein family, plays a crucial role. However, the specific functions and potential mechanisms of SRSF1 in advancing bladder cancer (BCa) progression and influencing chemosensitivity remain largely unexplored.
methodsThe expression of SRSF1 in BCa tissues and cell lines was investigated using quantitative real-time PCR (RT-qPCR) and western blotting. Survival analysis was employed to examine the association between SRSF1 expression and prognosis of BCa. The functions of SRSF1 were evaluated through proliferation assays, migration assays, IC50 determination assays, and tumorigenesis assays in nude mice. Subsequent RNA sequencing validated the relationship between SRSF1 alternative splicing and the mitophagy pathway. Mitochondrial membrane potential (MMP) was assessed using JC-1 staining. Mitophagy and autophagic flux were quantified using transmission electron microscopy and fluorescence imaging. RNA immunoprecipitation, CUT & RUN assays, and luciferase reporter assays were performed to validate the SRSF1/HIF1A/BNIP3 axis.
resultsHigh expression of SRSF1 in BCa was significantly associated with poor prognosis. SRSF1 promoted the progression of BCa cells and conferred resistance to cisplatin both in vitro and in vivo. Mechanistically, SRSF1 interacted with pre-HIF1A via the RRM1/RRM2 domain, thereby enhancing the production of the transcription factor HIF1A through the alternative splicing pathway. This interaction subsequently activated the HIF1A/BNIP3 axis, which promoted mitophagy in BCa. Ultimately, this led to further progression of bladder cancer and a decrease in cisplatin sensitivity.
conclusionsSRSF1 indicated poor prognosis and promoted the progression and cisplatin resistance of BCa cells through the HIF1A/BNIP3/mitophagy axis. It holds significant potential as a novel biomarker for the diagnosis and treatment of BCa, particularly in chemotherapy.
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