Evidence map›Paper›PMID 40405032›Full record

ArticlePlant cell reports2025

Designer circRNA

M Hossain, C Pfafenrot, S Nasfi, A Sede, J Imani, E Šečić, M Galli, P Schäfer, A Bindereif, M Heinlein and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Plant cell reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

M HossainInstitute of Phytopathology, Centre for BioSystems, Land Use and Nutrition, Justus Liebig University, Heinrich-Buff-Ring 26, 35392, Giessen, Germany.ORCID http://orcid.org/0009-0006-1528-4167
C PfafenrotInstitute of Biochemistry, Justus Liebig University, Heinrich-Buff-Ring 17, 35392, Giessen, Germany.ORCID http://orcid.org/0009-0009-1846-3613
S NasfiInstitute of Phytopathology, Centre for BioSystems, Land Use and Nutrition, Justus Liebig University, Heinrich-Buff-Ring 26, 35392, Giessen, Germany.ORCID http://orcid.org/0000-0001-5916-0824
A SedeInstitut de Biologie Moléculaire des Plantes, CNRS, Université de Strasbourg, 12 rue du Général Zimmer, 67084, Strasbourg, France.ORCID http://orcid.org/0000-0002-7868-0638
J ImaniInstitute of Phytopathology, Centre for BioSystems, Land Use and Nutrition, Justus Liebig University, Heinrich-Buff-Ring 26, 35392, Giessen, Germany.ORCID http://orcid.org/0000-0001-8265-1656
E ŠečićInstitute of Phytopathology, Centre for BioSystems, Land Use and Nutrition, Justus Liebig University, Heinrich-Buff-Ring 26, 35392, Giessen, Germany.ORCID http://orcid.org/0000-0002-6469-4895
M GalliInstitute of Phytopathology, Centre for BioSystems, Land Use and Nutrition, Justus Liebig University, Heinrich-Buff-Ring 26, 35392, Giessen, Germany.ORCID http://orcid.org/0000-0003-4514-9434
P SchäferInstitute of Phytopathology, Centre for BioSystems, Land Use and Nutrition, Justus Liebig University, Heinrich-Buff-Ring 26, 35392, Giessen, Germany.ORCID http://orcid.org/0000-0002-0366-6858
A BindereifInstitute of Biochemistry, Justus Liebig University, Heinrich-Buff-Ring 17, 35392, Giessen, Germany.ORCID http://orcid.org/0000-0002-7730-7380
M HeinleinInstitut de Biologie Moléculaire des Plantes, CNRS, Université de Strasbourg, 12 rue du Général Zimmer, 67084, Strasbourg, France.ORCID http://orcid.org/0000-0001-7322-1654
M Ladera-CarmonaInstitute of Phytopathology, Centre for BioSystems, Land Use and Nutrition, Justus Liebig University, Heinrich-Buff-Ring 26, 35392, Giessen, Germany.ORCID http://orcid.org/0000-0003-2167-3135
K H KogelInstitute of Phytopathology, Centre for BioSystems, Land Use and Nutrition, Justus Liebig University, Heinrich-Buff-Ring 26, 35392, Giessen, Germany. karl-heinz.kogel@uni-giessen.de.ORCID http://orcid.org/0000-0003-1226-003X

Funding

ANR ANR-21-SUSC-0003-01 to MHDeutsche Forschungsgemeinschaft 459501999Deutsche Forschungsgemeinschaft FOR5116
6 · The paper itself

Abstract

key messageWe demonstrate non-immunogenic circRNA as a tool for targeted gene regulation in plants, where it acts in an isoform- and sequence-specific manner, enabling future agronomic applications. Circular RNAs (circRNAs) are single-stranded RNA molecules characterized by their covalently closed structure and are emerging as key regulators of cellular processes in mammals, including gene expression, protein function and immune responses. Recent evidence suggests that circRNAs also play significant roles in plants, influencing development, nutrition, biotic stress resistance, and abiotic stress tolerance. However, the potential of circRNAs to modulate target protein abundance in plants remains largely unexplored. In this study, we investigated the potential of designer circRNAs to modulate target protein abundance in plants using Arabidopsis protoplasts as a model system. We show that PEG-mediated transfection with a 50-nt circRNA

Indexed as

ArabidopsisGreen Fluorescent ProteinsProtoplastsRNA, CircularRNA InterferenceArabidopsis ProteinsGene Expression Regulation, PlantArabidopsis ProteinsGreen Fluorescent ProteinsRNA, CircularCircular RNADisease resistanceDouble-stranded RNAProtoplast transfectionRNA interferenceSmall RNA

Identifiers

PMID40405032
PMCPMC12098445

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.