ArticleScientific reports2025
Photobiomodulation promotes osteogenic differentiation of mesenchymal stem cells and increases P-Akt levels in vitro.
Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Innovative photobiomodulation-enhanced T-PRF in posterior mandibular fractures: a randomized pilot study.BMC oral health · 2026Trial
- Reactive oxygen species drive wound epithelium and blastema formation in regenerating tail of Scincella tsinlingensis.Frontiers in zoology · 2026Article
- Review
- Photobiomodulation With 976 nm Diode Laser Enhances Osteoblastic Differentiation of Saos-2 Cells Cultured on Collagen Membranes.Clinical and experimental dental research · 2026Article
- Laser-Based Photobiomodulation in Postoperative Tissue Healing in Oral and Maxillofacial Surgery: Systematic Review of RCTs.Journal of clinical medicine · 2026Review
- Heterologous Fibrin Biopolymer as a Key Scaffold for Bone Regeneration: Synergistic Effects with Photobiomodulation and Membrane Therapy.Gels (Basel, Switzerland) · 2026Article
- A Concise Review of the Impact of Photobiomodulation (PBM) on Neuronal Differentiation in Adipose-Derived Mesenchymal Stem Cells.Journal of lasers in medical sciences · 2026Review
- Photobiomodulation stimulates mitochondrial function and cell proliferation in meniscus-derived stem cells (MeSCs) via activation of TRPV1 channel.Scientific reports · 2025Article
- Determination of Osteocalcin Levels in Gingival Crevicular Fluid to Assess the Effectiveness of Laser Therapy in Patients with Orthodontic Treatment.Biomedicines · 2025Article
- Impact of Photobiomodulation on the Pro-Osteogenic Activity of Dental Pulp Mesenchymal Stem/Stromal Cells.International journal of molecular sciences · 2025Article
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Authors and funding
7 authors.
Funding
Abstract
Bone defects are common orthopedic conditions, and due to the limited regenerative capacity of bone tissue, their repair remains a challenge in orthopedic surgery. Mesenchymal stem cells (MSCs) have demonstrated strong potential for osteogenic differentiation; however, their efficiency in vivo remains restricted, particularly in terms of differentiation and migration. Photobiomodulation (PBM), a non-invasive therapeutic technique, has shown great promise in promoting stem cell differentiation. In this study, we cultured human umbilical cord mesenchymal stem cells (hUCMSCs) in vitro and treated them with 635/808 nm laser light. We measured alkaline phosphatase (ALP) activity, mineralized nodule formation, and the expression of osteogenesis-related genes and factors after 7, 14, and 21 days. The results showed that PBM treatment significantly enhanced hUCMSC proliferation and osteogenic differentiation. The mechanism behind this effect involves PBM activating the upstream Akt signaling pathway, increasing P-Akt expression, and elevating reactive oxygen species (ROS) levels to induce mild oxidative stress. This process enhances ALP activity, mineralized nodule formation, and the expression of osteogenesis-related genes and factors, thus promoting the osteogenic differentiation of hUCMSCs.
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