ArticleAnalytical chemistry2025
Quantitative Mass Spectrometry Imaging Protocols for Spatially Heterogeneous Samples.
Article in Analytical chemistry, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed.
- A novel approach for monitoring the spatial distribution and quantitative analysis of micronutrients in plant tissues using laser ablation ICP-MS imaging.Analytical and bioanalytical chemistry · 2026Article
- Insights From Spatiality: Accelerating Pharmaceutical Research Using Mass Spectrometry Imaging.Medicinal research reviews · 2026Review
- Evaluating Batch Correction Methods for Large-Scale Mass Spectrometry Imaging of Heterogeneous Tissues.Analytical chemistry · 2026Article
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) is a promising tool for the spatial quantitation of endogenous and exogenous compounds directly in biological tissue sections. However, precise quantitation may be hampered due to matrix effects and variations in ionization efficiency, especially in spatially heterogeneous samples such as brain tissue. In this study, we developed and implemented two advanced MALDI-MSI protocols to address these limitations by employing a standard addition approach. The protocols involved the homogeneous spraying of standard solutions onto tissue sections to minimize the matrix effects associated with heterogeneous samples. The first method utilized spraying of deuterated analogues of neurotransmitters across all tissue sections for normalization, while calibration standards were applied in a quantitative manner to consecutive tissue sections. The second method employed two stable isotope-labeled compounds: one for calibration and the other for normalization. Both methods were applied to quantify neurotransmitters and their metabolites, e.g., dopamine, norepinephrine, and 3-methoxytyramine, in rodent brain tissue. The results showed strong linearity between signal intensities and analyte concentrations across brain tissue sections with values comparable to those obtained using high-performance liquid chromatography-electrochemical detection. The standard addition approach significantly enhanced the quantitation accuracy by accounting for tissue-specific matrix effects, providing a robust method for the spatial quantification of neurotransmitters in complex brain tissue environments.
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Registered trials
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