Evidence map›Paper›PMID 40403726›Full record

ArticleCell reports methods2025

An AAV capsid proposed as microglia-targeting directs genetic expression in forebrain excitatory neurons.

Wenhao Cao, Zhiqun Tan, Bereket T Berackey, Jason K Nguyen, Sara R Brown, Shiyang Du, Bin Lin, Qiao Ye, Magdalene Seiler, Todd C Holmes and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell reports methods, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Targeting Microglial Transcriptional Reprogramming as a Therapy Strategy for Alzheimer's Disease.BioEssays : news and reviews in molecular, cellular and developmental biology · 2026
    Review
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Wenhao CaoDepartment of Anatomy and Neurobiology, School of Medicine, University of California, Irvine, Irvine, CA 92697, USA.
Zhiqun TanDepartment of Anatomy and Neurobiology, School of Medicine, University of California, Irvine, Irvine, CA 92697, USA; Center for Neural Circuit Mapping, University of California, Irvine, Irvine, CA 92697, USA.
Bereket T BerackeyDepartment of Anatomy and Neurobiology, School of Medicine, University of California, Irvine, Irvine, CA 92697, USA.
Jason K NguyenDepartment of Anatomy and Neurobiology, School of Medicine, University of California, Irvine, Irvine, CA 92697, USA.
Sara R BrownDepartment of Anatomy and Neurobiology, School of Medicine, University of California, Irvine, Irvine, CA 92697, USA.
Shiyang DuDepartment of Anatomy and Neurobiology, School of Medicine, University of California, Irvine, Irvine, CA 92697, USA.
Bin LinDepartment of Anatomy and Neurobiology, School of Medicine, University of California, Irvine, Irvine, CA 92697, USA; Department of Physical Medicine & Rehabilitation, School of Medicine, University of California, Irvine, Irvine, CA 92697, USA.
Qiao YeDepartment of Anatomy and Neurobiology, School of Medicine, University of California, Irvine, Irvine, CA 92697, USA.
Magdalene SeilerDepartment of Anatomy and Neurobiology, School of Medicine, University of California, Irvine, Irvine, CA 92697, USA; Department of Physical Medicine & Rehabilitation, School of Medicine, University of California, Irvine, Irvine, CA 92697, USA.
Todd C HolmesDepartment of Physiology and Biophysics, School of Medicine, University of California, Irvine, Irvine, CA 92697, USA; Center for Neural Circuit Mapping, University of California, Irvine, Irvine, CA 92697, USA.
Xiangmin XuDepartment of Anatomy and Neurobiology, School of Medicine, University of California, Irvine, Irvine, CA 92697, USA; Department of Microbiology and Molecular Genetics, School of Medicine, University of California, Irvine, Irvine, CA 92697, USA; Center for Neural Circuit Mapping, University of California, Irvine, Irvine, CA 92697, USA. Electronic address: xiangmix@uci.edu.

Funding

UV to blue neuronal phototransduction mechanismsR35GM127102 · NIGMS · UNIVERSITY OF CALIFORNIA-IRVINE · PI Todd C Holmes · 2018 to 2026
$4.4M
UC Irvine Center for the production and distribution of cell-type-specific viral targeting reagentsU24MH133236 · NIMH · UNIVERSITY OF CALIFORNIA-IRVINE · PI FISHELL, GORDON J, XU, XIANGMIN · 2023 to 2025
$3.6M
Customized cleared tissue light-sheet fluorescence microscope for imaging large brain samplesS10MH124715 · NIMH · UNIVERSITY OF CALIFORNIA-IRVINE · PI XU, XIANGMIN · 2021 to 2021
$436k
NIGMS NIH HHS R35 GM127102NIMH NIH HHS S10 MH124715NIMH NIH HHS U24 MH133236
6 · The paper itself

Abstract

A newly developed capsid AAV-MG1.2 was reported to mediate specific microglial transduction. However, we find that AAV-MG1.2 actually enables specific genetic access to excitatory neurons in forebrain regions including hippocampal formation and visual cortex but does not confer expression in microglia or astrocytes in vivo. Furthermore, we find that AAV-MG1.2 specifically labels the deep layer of the CA1 pyramidal layer in a titer-dependent manner. We show that AAV-MG1.2-Cre can be used to genetically target excitatory neurons for cell-type-specific neural circuit mapping studies. We also find that AAV-MG1.2 conserves specificity for excitatory neurons in rat hippocampus. Thus, the AAV-MG1.2 presents a useful viral-genetic tool for targeting excitatory neurons in the forebrain across different species.

Indexed as

CapsidDependovirusMicrogliaNeuronsProsencephalonAnimalsGene ExpressionGenetic VectorsHippocampusMaleMiceRatsTransduction, GeneticAAV-MG1.2AAV toolboxCP: neurosciencecross-speciesexcitatory neuronshippocampusneocortexneural circuit tracingtool development

Identifiers

PMID40403726
PMCPMC12272241

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.