Evidence map›Paper›PMID 40403707›Full record

ArticleNeuron2025

Specific targeting of brain endothelial cells using enhancer AAV vectors.

Eric Velazquez-Rivera, Oyshi Dey, Nayoon S Kim, Wenhao Cao, Qiao Ye, Pan Gao, Andy Thai, Jason K Nguyen, Hai Zhang, Jonathan T Ting and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Neuron, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed.

  1. Article
  2. Article
  3. Towards precision medicine for brain arteriovenous malformations.The Journal of clinical investigation · 2026
    Review
  4. Review
  5. Defining a Midgestational Window forbioRxiv : the preprint server for biology · 2026
    Article
  6. Article
  7. Review
  8. Combining Machine Learning and Multiplexed,bioRxiv : the preprint server for biology · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Eric Velazquez-RiveraDepartment of Anatomy and Neurobiology, School of Medicine, University of California, Irvine, Irvine, CA 92697, USA.
Oyshi DeyDepartment of Anatomy and Neurobiology, School of Medicine, University of California, Irvine, Irvine, CA 92697, USA.
Nayoon S KimDepartment of Anatomy and Neurobiology, School of Medicine, University of California, Irvine, Irvine, CA 92697, USA.
Wenhao CaoDepartment of Anatomy and Neurobiology, School of Medicine, University of California, Irvine, Irvine, CA 92697, USA.
Qiao YeDepartment of Anatomy and Neurobiology, School of Medicine, University of California, Irvine, Irvine, CA 92697, USA.
Pan GaoDepartment of Anatomy and Neurobiology, School of Medicine, University of California, Irvine, Irvine, CA 92697, USA.
Andy ThaiDepartment of Computer Science, University of California, Irvine, Irvine, CA 92697, USA.
Jason K NguyenDepartment of Anatomy and Neurobiology, School of Medicine, University of California, Irvine, Irvine, CA 92697, USA.
Hai ZhangDepartment of Anatomy and Neurobiology, School of Medicine, University of California, Irvine, Irvine, CA 92697, USA.
Jonathan T TingHuman Cell Types Program, Allen Institute for Brain Science, Seattle, WA 98109, USA.
M GopiDepartment of Computer Science, University of California, Irvine, Irvine, CA 92697, USA.
Bing RenDepartment of Cellular and Molecular Medicine, School of Medicine, University of California, San Diego, La Jolla, CA 92093, USA; Center for Neural Circuit Mapping (CNCM), University of California, Irvine, Irvine, CA 92697, USA.
Todd C HolmesDepartment of Physiology and Biophysics, School of Medicine, University of California, Irvine, Irvine, CA 92697, USA; Center for Neural Circuit Mapping (CNCM), University of California, Irvine, Irvine, CA 92697, USA.
Xiangmin XuDepartment of Anatomy and Neurobiology, School of Medicine, University of California, Irvine, Irvine, CA 92697, USA; Department of Computer Science, University of California, Irvine, Irvine, CA 92697, USA; Department of Biomedical Engineering, University of California, Irvine, Irvine, CA 92697, USA; Department of Microbiology and Molecular Genetics, University of California, Irvine, Irvine, CA 92697, USA; Center for Neural Circuit Mapping (CNCM), University of California, Irvine, Irvine, CA 92697, USA. Electronic address: xiangmix@hs.uci.edu.

Funding

Cell-type-specific neural circuit connectomes in the mouse models of aging and Alzheimer's diseaseU01AG076791 · NIA · UNIVERSITY OF CALIFORNIA-IRVINE · PI Kim Green, ERAN A MUKAMEL · 2022 to 2026
$13.6M
UV to blue neuronal phototransduction mechanismsR35GM127102 · NIGMS · UNIVERSITY OF CALIFORNIA-IRVINE · PI Todd C Holmes · 2018 to 2026
$4.4M
UC Irvine Center for the production and distribution of cell-type-specific viral targeting reagentsU24MH133236 · NIMH · UNIVERSITY OF CALIFORNIA-IRVINE · PI FISHELL, GORDON J, XU, XIANGMIN · 2023 to 2025
$3.6M
FDA HHS R01 FD007478NIA NIH HHS U01 AG076791NIGMS NIH HHS R35 GM127102NIMH NIH HHS U24 MH133236
6 · The paper itself

Abstract

Brain endothelial cells (BECs) in brain vasculature are critical structural and functional components of the blood brain barrier (BBB). Adeno-associated virus (AAV) capsids have previously been genetically engineered to confer specificity to endothelial cells, but these capsids show limited endothelial cell specificity that varies by delivery conditions. We developed a set of new BEC-enhancer AAV vectors that specifically target BECs based on the cis-regulatory elements identified from single-cell epigenetic datasets. Ex vivo and in vivo characterization of BEC-enhancer AAVs in wild-type, Ai9 reporter, and Alzheimer's disease model mouse brains show their utility for high transduction selectivity of the BECs with little off-target transduction in the liver. Our BEC-enhancer AAVs target the brain vasculature by systemic administration and can be minimally invasive in terms of the route of administration. They are useful new tools for delivering genetic payloads specifically to BECs for normal and diseased brain studies.

Indexed as

Blood-Brain BarrierBrainDependovirusEndothelial CellsEnhancer Elements, GeneticGenetic VectorsAlzheimer DiseaseAnimalsHumansMiceMice, Inbred C57BLTransduction, GeneticAAVAlzheimer'sendothelial cellsenhancergene therapygenetic accessrecombinase

Identifiers

PMID40403707
PMCPMC12117388

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.