ArticleNeuron2025
An enhancer-AAV toolbox to target and manipulate distinct interneuron subtypes.
Abstract read
In one paragraph
Article in Neuron, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
0numbers the graph read from it
0cells of the map it votes in
48citing papers in PubMed
–field-weighted citation impact
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
48 citing papers in PubMed.
- GluD1 is localized at cholinergic synapses and is an acetylcholine receptor.Molecular psychiatry · 2026Article
- GABAergic subiculum-medial entorhinal projection in mice and rats.Cerebral cortex (New York, N.Y. : 1991) · 2026Article
- Article
- Targeting the peripheral nervous system in adipose tissue with engineered AAV vectors.Cell reports methods · 2026Article
- AAV-mediated gene therapy demonstrates phenotypic rescue in a mouse model of Cockayne syndrome.The Journal of clinical investigation · 2026Article
- Article
- Learning how to experience the world: From circuits to cell types to genes.Current opinion in neurobiology · 2026Review
- TRACR: an anterograde transneuronal tracing system for genetic access across synapses and longitudinal circuit analysis.bioRxiv : the preprint server for biology · 2026Article
- Resting fMRI functional connectivity reflects fluctuations in inhibitory interneuron activity.bioRxiv : the preprint server for biology · 2026Article
- Surgical protocol for precise and high-throughput viral injections in rhesus monkey brain.STAR protocols · 2026Article
- Article
- Context-dependent effects of SOM neurons to enhance the responses of corticocollicular neurons to repetitive sounds.iScience · 2026Article
- Technical and biological sources of noise confound multiplexed enhancer AAV screening.Nature communications · 2026Article
- CBLN2 promoter enables genetic access to wide-field neurons of the tree shrew superior colliculus.Cell reports methods · 2026Article
- Multimodal characterization of transcriptionally defined ventral tegmental area dopamine neurons.bioRxiv : the preprint server for biology · 2026Article
- Multi-omic profiling of human and mouse dorsal root ganglia enables targeted gene delivery to nociceptors.bioRxiv : the preprint server for biology · 2026Article
- AAV-Based Bright and Sparse Labeling of Versatile Neurons Adaptable in Cre-Dependent Genetic Backgrounds.eNeuro · 2026Article
- Subthreshold Kir and IBiological research · 2026Article
- Developmental dysregulation of chandelier cell excitability in a mouse model of Dravet Syndrome.bioRxiv : the preprint server for biology · 2026Article
- AAV-PHP.eB Peripheral Delivery and Central Expression in Cre Mice.The Journal of comparative neurology · 2026Article
Corrections and comments
- Update of
Authors and funding
71 authors.
Elisabetta FurlanisHarvard Medical School, Blavatnik Institute, Department of Neurobiology, Boston, MA 02115, USA; Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
Min DaiHarvard Medical School, Blavatnik Institute, Department of Neurobiology, Boston, MA 02115, USA; Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
Brenda Leyva GarciaHarvard Medical School, Blavatnik Institute, Department of Neurobiology, Boston, MA 02115, USA; Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
Thien TranHarvard Medical School, Blavatnik Institute, Department of Neurobiology, Boston, MA 02115, USA; Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
Josselyn VergaraHarvard Medical School, Blavatnik Institute, Department of Neurobiology, Boston, MA 02115, USA; Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
Ana PereiraHarvard Medical School, Blavatnik Institute, Department of Neurobiology, Boston, MA 02115, USA; Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
Bram L GorissenHarvard Medical School, Blavatnik Institute, Department of Neurobiology, Boston, MA 02115, USA; Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
Sara WillsHarvard Medical School, Blavatnik Institute, Department of Neurobiology, Boston, MA 02115, USA; Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
Anna VlachosEunice Kennedy Shriver National Institute of Child Health and Human Development, NIH, Bethesda, MD 20892, USA.
Ariel HairstonHarvard Medical School, Blavatnik Institute, Department of Neurobiology, Boston, MA 02115, USA.
Deepanjali DwivediHarvard Medical School, Blavatnik Institute, Department of Neurobiology, Boston, MA 02115, USA; Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
Sarah DuHarvard Medical School, Blavatnik Institute, Department of Neurobiology, Boston, MA 02115, USA; Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
Justin McMahonHarvard Medical School, Blavatnik Institute, Department of Neurobiology, Boston, MA 02115, USA; Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
Shuhan HuangHarvard Medical School, Blavatnik Institute, Department of Neurobiology, Boston, MA 02115, USA.
Annunziato MorabitoHarvard Medical School, Blavatnik Institute, Department of Neurobiology, Boston, MA 02115, USA.
Arenski VazquezDominick P. Purpura Department of Neuroscience, Albert Einstein College of Medicine, Bronx, NY, USA.
Soyoun KimDominick P. Purpura Department of Neuroscience, Albert Einstein College of Medicine, Bronx, NY, USA.
Anthony T LeeDepartment of Neurological Surgery, University of California, San Francisco, San Francisco, CA, USA; Weill Institute for Neurosciences, University of California, San Francisco, San Francisco, CA, USA.
Edward F ChangDepartment of Neurological Surgery, University of California, San Francisco, San Francisco, CA, USA; Weill Institute for Neurosciences, University of California, San Francisco, San Francisco, CA, USA.
Taha RazzaqTibbling Technologies, Redmond, WA, USA.
Ahmed QaziTibbling Technologies, Redmond, WA, USA.
Geoffrey VargishEunice Kennedy Shriver National Institute of Child Health and Human Development, NIH, Bethesda, MD 20892, USA.
Xiaoqing YuanEunice Kennedy Shriver National Institute of Child Health and Human Development, NIH, Bethesda, MD 20892, USA.
Adam CaccavanoEunice Kennedy Shriver National Institute of Child Health and Human Development, NIH, Bethesda, MD 20892, USA.
Steven HuntEunice Kennedy Shriver National Institute of Child Health and Human Development, NIH, Bethesda, MD 20892, USA.
Ramesh ChittajalluEunice Kennedy Shriver National Institute of Child Health and Human Development, NIH, Bethesda, MD 20892, USA.
Nadiya McLeanEunice Kennedy Shriver National Institute of Child Health and Human Development, NIH, Bethesda, MD 20892, USA.
Lauren HewittEunice Kennedy Shriver National Institute of Child Health and Human Development, NIH, Bethesda, MD 20892, USA.
Emily ParanzinoEunice Kennedy Shriver National Institute of Child Health and Human Development, NIH, Bethesda, MD 20892, USA.
Haley RiceEunice Kennedy Shriver National Institute of Child Health and Human Development, NIH, Bethesda, MD 20892, USA.
Alex C CumminsNational Institute of Mental Health, National Institutes of Health, Bethesda, MD 20892-4415, USA.
Anya PlotnikovaNational Institute of Mental Health, National Institutes of Health, Bethesda, MD 20892-4415, USA.
Arya MohantyNational Institute of Mental Health, National Institutes of Health, Bethesda, MD 20892-4415, USA.
Anne Claire TangenNational Institute of Mental Health, National Institutes of Health, Bethesda, MD 20892-4415, USA.
Jung Hoon ShinNational Institute of Mental Health, National Institutes of Health, Bethesda, MD 20892-4415, USA.
Reza AzadiNational Institute of Mental Health, National Institutes of Health, Bethesda, MD 20892-4415, USA.
Mark A G EldridgeNational Institute of Mental Health, National Institutes of Health, Bethesda, MD 20892-4415, USA.
Veronica A AlvarezNational Institute of Mental Health, National Institutes of Health, Bethesda, MD 20892-4415, USA.
Bruno B AverbeckNational Institute of Mental Health, National Institutes of Health, Bethesda, MD 20892-4415, USA.
Mansour AlyahyayBiological and Environmental Sciences and Engineering Division, King Abdullah University of Science and Technology, Thuwal 23955-6900, Kingdom of Saudi Arabia.
Tania Reyes VallejoBiological and Environmental Sciences and Engineering Division, King Abdullah University of Science and Technology, Thuwal 23955-6900, Kingdom of Saudi Arabia.
Mohammed SoheibBiological and Environmental Sciences and Engineering Division, King Abdullah University of Science and Technology, Thuwal 23955-6900, Kingdom of Saudi Arabia.
Lucas G VattinoEaton-Peabody Laboratories, Massachusetts Eye and Ear Infirmary, Boston, MA, USA; Department of Otolaryngology - Head and Neck Surgery, Harvard Medical School, Boston, MA, USA.
Cathryn P MacGregorEaton-Peabody Laboratories, Massachusetts Eye and Ear Infirmary, Boston, MA, USA; Department of Otolaryngology - Head and Neck Surgery, Harvard Medical School, Boston, MA, USA.
Carolina Piletti ChatainDepartment of Neuroscience, Kavli Institute for Neuroscience, Yale University School of Medicine, New Haven, CT 06510, USA; Wu Tsai Institute, Yale University, New Haven, CT 06510, USA.
Emmie BanksDepartment of Cell Biology, Emory University School of Medicine, Atlanta, GA 30322, USA.
Viktor Janos OlahDepartment of Cell Biology, Emory University School of Medicine, Atlanta, GA 30322, USA.
Shovan NaskarUnit of Functional Neural Circuit, National Institute of Mental Health, National Institutes of Health, Bethesda, MD 20892, USA.
Sophie HillDepartment of Neuroscience, The University of Pennsylvania Perelman School of Medicine, Philadelphia, PA 19104, USA.
Sophie LiebergallDepartment of Neuroscience, The University of Pennsylvania Perelman School of Medicine, Philadelphia, PA 19104, USA.
Rohan BadianiHarvard Medical School, Blavatnik Institute, Department of Neurobiology, Boston, MA 02115, USA; Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
Lili HydeHarvard Medical School, Blavatnik Institute, Department of Neurobiology, Boston, MA 02115, USA; Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
Ella HanleyHarvard Medical School, Blavatnik Institute, Department of Neurobiology, Boston, MA 02115, USA; Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
Qing XuBiological and Environmental Sciences and Engineering Division, King Abdullah University of Science and Technology, Thuwal 23955-6900, Kingdom of Saudi Arabia; Genomics & Systems Biology, New York University Abu Dhabi, Abu Dhabi, UAE.
Kathryn C AllawayHarvard Medical School, Blavatnik Institute, Department of Neurobiology, Boston, MA 02115, USA; Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
Ethan M GoldbergDepartment of Neuroscience, The University of Pennsylvania Perelman School of Medicine, Philadelphia, PA 19104, USA.
Matthew J M RowanDepartment of Cell Biology, Emory University School of Medicine, Atlanta, GA 30322, USA.
Tomasz J NowakowskiDepartment of Neurological Surgery, University of California, San Francisco, San Francisco, CA, USA; Weill Institute for Neurosciences, University of California, San Francisco, San Francisco, CA, USA.
Soohyun LeeUnit of Functional Neural Circuit, National Institute of Mental Health, National Institutes of Health, Bethesda, MD 20892, USA.
Emilia FavuzziDepartment of Neuroscience, Kavli Institute for Neuroscience, Yale University School of Medicine, New Haven, CT 06510, USA; Wu Tsai Institute, Yale University, New Haven, CT 06510, USA.
Pascal S KaeserHarvard Medical School, Blavatnik Institute, Department of Neurobiology, Boston, MA 02115, USA.
Lucas SjulsonDominick P. Purpura Department of Neuroscience, Albert Einstein College of Medicine, Bronx, NY, USA; Dominick P. Purpura Department of Psychiatry and Behavioral Sciences, Albert Einstein College of Medicine, Bronx, NY, USA.
Renata Batista-BritoDominick P. Purpura Department of Neuroscience, Albert Einstein College of Medicine, Bronx, NY, USA; Dominick P. Purpura Department of Psychiatry and Behavioral Sciences, Albert Einstein College of Medicine, Bronx, NY, USA.
Anne E TakesianEaton-Peabody Laboratories, Massachusetts Eye and Ear Infirmary, Boston, MA, USA; Department of Otolaryngology - Head and Neck Surgery, Harvard Medical School, Boston, MA, USA.
Leena A IbrahimBiological and Environmental Sciences and Engineering Division, King Abdullah University of Science and Technology, Thuwal 23955-6900, Kingdom of Saudi Arabia.
Asim IqbalTibbling Technologies, Redmond, WA, USA.
Kenneth A PelkeyEunice Kennedy Shriver National Institute of Child Health and Human Development, NIH, Bethesda, MD 20892, USA.
Chris J McBainEunice Kennedy Shriver National Institute of Child Health and Human Development, NIH, Bethesda, MD 20892, USA.
Jordane DimidschsteinStanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA. Electronic address: jordane.dimidschstein@gmail.com.
Gord FishellHarvard Medical School, Blavatnik Institute, Department of Neurobiology, Boston, MA 02115, USA; Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA. Electronic address: gfishell@broadinstitute.org.
Yating WangHarvard Medical School, Blavatnik Institute, Department of Neurobiology, Boston, MA 02115, USA; Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA. Electronic address: yawang@broadinstitute.org.
Funding
SUPPORT FOR THE ROSE F KENNEDY IDDRC P50P50HD105352 · NICHD · ALBERT EINSTEIN COLLEGE OF MEDICINE · PI Julie Secombe · 2021 to 2026
$7.0MNICHD NIH HHS P50 HD105352
Abstract
In recent years, we and others have identified a number of enhancers that, when incorporated into rAAV vectors, can restrict the transgene expression to particular neuronal populations. Yet, viral tools to access and manipulate specific neuronal subtypes are still limited. Here, we performed systematic analysis of single-cell genomic data to identify enhancer candidates for each of the telencephalic interneuron subtypes. We established a set of enhancer-AAV tools that are highly specific for distinct cortical interneuron populations and striatal cholinergic interneurons. These enhancers, when used in the context of different effectors, can target (fluorescent proteins), observe activity (GCaMP), and manipulate (opto-genetics) specific neuronal subtypes. We also validated our enhancer-AAV tools across species. Thus, we provide the field with a powerful set of tools to study neural circuits and functions and to develop precise and targeted therapy.
Indexed as
DependovirusEnhancer Elements, GeneticInterneuronsAnimalsCorpus StriatumGenetic VectorsMiceadeno-associated viruscholinergiccortexenhancerGABAergicinterneuron
Identifiers
PMID
40403705What OpenQuestion holds
Textmetadata
Read underepoch 390
Registered trials
None linked
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.