Epigenomic Profiling of Histone Posttranslational Modifications in Frozen Cancer Tissue Using CUT&RUN.

Annalisa Roberti, Mario Fernández Fraga

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Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

2 authors.

Annalisa RobertiCancer Epigenetics and Nanomedicine Laboratory, Nanomaterials and Nanotechnology Research Center (CINN), Spanish National Research Council (CSIC), El Entrego, Spain.

Mario Fernández FragaCancer Epigenetics and Nanomedicine Laboratory, Nanomaterials and Nanotechnology Research Center (CINN), Spanish National Research Council (CSIC), El Entrego, Spain. mffraga@cinn.es.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Alterations in the epigenetic landscape are now recognized as a hallmark of cancer. Among these, histone posttranslational modifications (HPTMs) represent a critical control layer, playing a significant role in chromatin remodeling and gene expression regulation. The comprehensive mapping of these modifications on a genome-wide scale is crucial for identifying regulatory regions and understanding downstream pathways, thereby providing a deeper and more integrated understanding of cancer biology. CUT&RUN (Cleavage Under Targets and Release Using Nuclease), combined with next-generation sequencing, is emerging as a highly reliable, low-background, and user-friendly technology for mapping antibody-bound chromatin regions in a genome-wide manner. In this chapter, we provide a comprehensive overview of an optimized protocol for targeting HPTMs in frozen tissue samples, emphasizing the crucial role of nucleus purification in our methodology.

Indexed as

Epigenesis, GeneticEpigenomicsHistonesNeoplasmsProtein Processing, Post-TranslationalChromatinHigh-Throughput Nucleotide SequencingHumansChromatinHistonesCUT&RUNFrozen cancer tissueGenome-wide mappingHistone posttranslational modificationsNuclei purification

Identifiers

PMID40402458

DOI10.1016/j.molcel.2021.03.045

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.