Evidence map›Paper›PMID 40402456›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Chromatin Preparation from Frozen Tissues for Chromatin Immunoprecipitation (ChIP) Assays.

Virginia López Martínez, Mario Fernández Fraga

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Virginia López MartínezCancer Epigenetics and Nanomedicine Laboratory, Centro de Investigación en Nanomateriales y Nanotecnología-Consejo Superior de Investigaciones Científicas (CINN-CSIC), Instituto de Investigación Sanitaria del Principado de Asturias (ISPA-FINBA), Centro de Investigación Biomédica en Red de Enfermedades Raras (CIBERER), Instituto de Salud Carlos III (ISCIII), Instituto Universitario de Oncología del Principado de Asturias (IUOPA), Universidad de Oviedo, Oviedo, Spain.
Mario Fernández FragaCancer Epigenetics and Nanomedicine Laboratory, Centro de Investigación en Nanomateriales y Nanotecnología-Consejo Superior de Investigaciones Científicas (CINN-CSIC), Instituto de Investigación Sanitaria del Principado de Asturias (ISPA-FINBA), Centro de Investigación Biomédica en Red de Enfermedades Raras (CIBERER), Instituto de Salud Carlos III (ISCIII), Instituto Universitario de Oncología del Principado de Asturias (IUOPA), Universidad de Oviedo, Oviedo, Spain. mffraga@cinn.es.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Chromatin immunoprecipitation coupled with next-generation sequencing (ChIP-seq) is a crucial method for examining transcription factor binding and histone modifications across the entire genome. This is a key step in deciphering the complex mechanisms that control cancer immunosurveillance. Here, we provide a comprehensive protocol covering all the steps needed to obtain DNA for ChIP-seq library preparation, starting from tissue dissection, followed by fixation, chromatin preparation, immunoprecipitation, and finally DNA purification. The protocol is optimized for frozen mice tissues, but can be easily adapted for use with any model organism. The resulting immunoprecipitated chromatin is suitable for library preparation and sequencing on an Illumina platform.

Indexed as

ChromatinChromatin ImmunoprecipitationChromatin Immunoprecipitation SequencingAnimalsDNAGene LibraryHigh-Throughput Nucleotide SequencingMiceTissue FixationChromatinDNAChromatin immunoprecipitationEpigenomic profilingFrozen tissuesSonication

Identifiers

PMID40402456

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.