ArticleAMB Express2025
Enhanced production and purification of L-asparaginase from Bacillus paralicheniformis AUMC B-516 with potent cytotoxicity against MCF-7 cell lines.
Article in AMB Express, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
2 citing papers in PubMed.
- Fungal L-Asparaginase from environmental isolates with antimicrobial and antibiofilm activities.AMB Express · 2026Article
- Submerged Fermentation ofACS omega · 2025Article
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
L-asparaginase is an important enzyme used in the pharmaceutical and food industries. Nowadays, bacterial species represent the main source of microbial synthesis for L-asparaginase. But studies aimed at improving production yields and new methods that use various microbes to expand the scope of application for the generated enzyme are also necessary for industrial manufacturing. This study focused on the isolation of Bacillus paralicheniformis AUMC B-516 and the optimization of L-asparaginase production under submerged fermentation. The enzyme was purified and characterized, followed by an evaluation of its cytotoxic effects against the MCF-7 human breast cancer cell line. The results revealed potent anticancer activity, highlighting the potential application of the purified enzyme in cancer therapeutics. Bacillus paralicheniformis AUMC B-516 was utilized for the biosynthesis of L-asparaginase (116.4 U/mL) after 48 h in the presence of 0.2% glucose and 1.0% L-asparagine at 35 °C and pH 8.0. Two-step chromatography (DEAE-cellulose and Sephacryl S200 HR) achieved a 12-fold purification, resulting in an enzyme specific activity of 4087.6 U/mg. For pure L-asparaginase that contained L-asparagine, the Km and Vmax values were 6.22 × 10
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