Evidence map›Paper›PMID 40402260›Full record

ArticleArchives of microbiology2025

One-step narrow-thermal-cycling strand exchange amplification for sensitive detection of porcine reproductive and respiratory syndrome virus.

Linlin Zhuang, Chunlei Song, Li Sun, Haiqiang Xie, Liqun Wang, Qingxin Liu, Hongjing Shi, Jianbo Yang, Qiuping Shen

Abstract read
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In one paragraph

Article in Archives of microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Linlin ZhuangSchool of Animal Husbandry and Veterinary Medicine, Jiangsu Vocational College of Agriculture and Forestry, Jurong, 212400, P. R. China.
Chunlei SongSchool of Animal Husbandry and Veterinary Medicine, Jiangsu Vocational College of Agriculture and Forestry, Jurong, 212400, P. R. China.
Li SunSchool of Animal Husbandry and Veterinary Medicine, Jiangsu Vocational College of Agriculture and Forestry, Jurong, 212400, P. R. China.
Haiqiang XieSchool of Animal Husbandry and Veterinary Medicine, Jiangsu Vocational College of Agriculture and Forestry, Jurong, 212400, P. R. China.
Liqun WangSchool of Animal Husbandry and Veterinary Medicine, Jiangsu Vocational College of Agriculture and Forestry, Jurong, 212400, P. R. China.
Qingxin LiuSchool of Animal Husbandry and Veterinary Medicine, Jiangsu Vocational College of Agriculture and Forestry, Jurong, 212400, P. R. China.
Hongjing ShiYangzhou Jianong Animal Husbandry Technology Co., Ltd, Yangzhou, 225251, P. R. China.
Jianbo YangSchool of Animal Husbandry and Veterinary Medicine, Jiangsu Vocational College of Agriculture and Forestry, Jurong, 212400, P. R. China. smzsyang@126.com.
Qiuping ShenSchool of Animal Husbandry and Veterinary Medicine, Jiangsu Vocational College of Agriculture and Forestry, Jurong, 212400, P. R. China. qiupingshen@outlook.com.

Funding

Natural Science Fund Project of Colleges in Jiangsu Province 22KJB180001Natural Science Fund Project of Jiangsu Vocational College of Agriculture and Forestry 2022kj32Natural Science Fund Project of Jiangsu Vocational College of Agriculture and Forestry 2023kj08
6 · The paper itself

Abstract

Porcine reproductive and respiratory syndrome (PRRS), which is primarily characterized by respiratory and reproductive dysfunction, is an epidemic disease caused by porcine reproductive and respiratory syndrome virus (PRRSV) that has the potential to economically devastate the global swine industry. Rapid and accurate detection of PRRSV is critical for effective control of PRRS in swine. In this study, a novel one-step, highly sensitive and specific accelerated strand exchange amplification (ASEA) method for the detection of PRRSV was developed. The detection limit of the ASEA method was determined to be 6 copies µL

Indexed as

Nucleic Acid Amplification TechniquesPorcine Reproductive and Respiratory SyndromePorcine respiratory and reproductive syndrome virusAnimalsRNA, ViralSensitivity and SpecificitySwineRNA, ViralPorcine reproductive and respiratory syndromePorcine reproductive and respiratory syndrome virusRapid detectionStrand exchange amplification

Identifiers

PMID40402260

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.