Evidence map›Paper›PMID 40401984›Full record

ArticleGlycobiology2025

GlycoSiteMiner: an ML/AI-assisted literature mining-based pipeline for extracting glycosylation sites from PubMed abstracts.

Robel Kahsay, Urnisha Bhuiyan, Cyrus Chun Hong Au, Nathan Edwards, Luke Johnson, Sujeet Kulkarni, Karina Martinez, Rene Ranzinger, K Vijay-Shanker, Jeet Vora and 3 more

Abstract read
In one paragraph

Article in Glycobiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Precision Profiling of the Cardiovascular Post-Translationally Modified Proteome.Journal of cardiovascular development and disease · 2026
    Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Robel KahsayDepartment of Biochemistry & Molecular Medicine, The George Washington School of Medicine and Health Sciences, 2300 Eye Street NW, Washington, DC 20052, United States.
Urnisha BhuiyanDepartment of Biochemistry & Molecular Medicine, The George Washington School of Medicine and Health Sciences, 2300 Eye Street NW, Washington, DC 20052, United States.
Cyrus Chun Hong AuDepartment of Biochemistry & Molecular Medicine, The George Washington School of Medicine and Health Sciences, 2300 Eye Street NW, Washington, DC 20052, United States.
Nathan EdwardsDepartment of Biochemistry and Molecular & Cellular Biology, 37th and O Street NW. Georgetown University, Washington, DC 20007, United States.
Luke JohnsonDepartment of Biochemistry & Molecular Medicine, The George Washington School of Medicine and Health Sciences, 2300 Eye Street NW, Washington, DC 20052, United States.
Sujeet KulkarniComplex Carbohydrate Research Center, The University of Georgia, 314 Riverbend Rd, Athens, GA 30602, United States.
Karina MartinezDepartment of Biochemistry & Molecular Medicine, The George Washington School of Medicine and Health Sciences, 2300 Eye Street NW, Washington, DC 20052, United States.
Rene RanzingerComplex Carbohydrate Research Center, The University of Georgia, 314 Riverbend Rd, Athens, GA 30602, United States.
K Vijay-ShankerDepartment of Computer and Information Sciences, University of Delaware, 18 Amstel Ave, Newark, DE 19716, United States.
Jeet VoraDepartment of Biochemistry & Molecular Medicine, The George Washington School of Medicine and Health Sciences, 2300 Eye Street NW, Washington, DC 20052, United States.
Kate WarnerDepartment of Biochemistry & Molecular Medicine, The George Washington School of Medicine and Health Sciences, 2300 Eye Street NW, Washington, DC 20052, United States.
Michael TiemeyerComplex Carbohydrate Research Center, The University of Georgia, 314 Riverbend Rd, Athens, GA 30602, United States.
Raja MazumderDepartment of Biochemistry & Molecular Medicine, The George Washington School of Medicine and Health Sciences, 2300 Eye Street NW, Washington, DC 20052, United States.

Funding

GlyGen growth and evolution into a central resource for glycans and glycoconjugatesR24GM146616 · NIGMS · UNIVERSITY OF GEORGIA · PI Raja Mazumder, Rene Ranzinger · 2022 to 2026
$5.5M
Harmonization of GlyGen glycoconjugate and glycan array data for integration into CFDEOT2OD032092 · OD · GEORGE WASHINGTON UNIVERSITY · PI MAZUMDER, RAJA, RANZINGER, RENE · 2021 to 2025
$3.1M
National Institutes of Health Office of Strategic Coordination 1OT2OD032092NIGMS NIH HHS 1R24GM146616NIGMS NIH HHS R24 GM146616NIH HHS OT2 OD032092
6 · The paper itself

Abstract

Over 50% of human proteins are estimated to be glycosylated, making glycosylation one of the most common post-translational modifications (PTMs) of proteins. A glycoinformatics resource such as the GlyGen knowledgebase, consisting of experimentally verified sequence-specific glycosylation sites, is critical for advancing research in glycobiology. Unfortunately, most experimental studies report glycosylation sites in free text format in scientific literature, mentioning gene names and amino acid positions without providing protein sequence identifiers, making it difficult to mine reported sites that can be mapped onto specific protein sequences. We have developed GlycoSiteMiner, which is an automated literature mining-based pipeline that extracts experimentally verified protein sequence-specific glycosylation sites from PubMed abstracts. The pipeline employs ML/AI algorithms to filter out incorrectly identified sites and has been applied to 33 million PubMed abstracts, identifying 1118 new sequence-specific glycosylation sites that were not previously present in the GlyGen resource.

Indexed as

Data MiningPubMedSoftwareAlgorithmsGlycosylationHumansProtein Processing, Post-TranslationalglycosylationGlyGenMLPPubTatorSVM

Identifiers

PMID40401984
PMCPMC12130968

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.