Evidence map›Paper›PMID 40401978›Full record

ArticleMicrobiology spectrum2025

Comprehensive evaluation of the MeltPro MTB/PZA assay for prediction of pyrazinamide resistance in multidrug-resistant tuberculosis.

Guiqing He, Chunxia Tu, Yelei Zhu, Qingyong Zheng, Qiulong Zhou, Wenzhen Zhou, Ouyang Huang, Bin Chen, Zhengwei Liu, Ye Xu and 1 more

Abstract readEvaluation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Microbiology spectrum, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Guiqing He *Department of Infectious Diseases, Wenzhou Central Hospital, The Dingli Clinical College of Wenzhou Medical University, Wenzhou, China.ORCID 0000-0002-6700-6920
Chunxia Tu *Engineering Research Centre of Molecular Diagnostics of the Ministry of Education, State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Faculty of Medicine and Life Sciences, Xiamen University, Xiamen, Fujian, China.ORCID 0009-0005-8829-7180
Yelei Zhu *Department of Tuberculosis Control and Prevention, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, China.ORCID 0000-0002-0781-7967
Qingyong ZhengLaboratory of Infectious Diseases, Wenzhou Central Hospital, The Dingli Clinical College of Wenzhou Medical University, Wenzhou, China.
Qiulong ZhouEngineering Research Centre of Molecular Diagnostics of the Ministry of Education, State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Faculty of Medicine and Life Sciences, Xiamen University, Xiamen, Fujian, China.
Wenzhen ZhouDepartment of Infectious Diseases, Wenzhou Central Hospital, The Dingli Clinical College of Wenzhou Medical University, Wenzhou, China.
Ouyang HuangDepartment of Infectious Diseases, Wenzhou Central Hospital, The Dingli Clinical College of Wenzhou Medical University, Wenzhou, China.
Bin ChenDepartment of Tuberculosis Control and Prevention, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, China.
Zhengwei LiuDepartment of Tuberculosis Control and Prevention, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, China.ORCID 0000-0002-5139-659X
Ye XuEngineering Research Centre of Molecular Diagnostics of the Ministry of Education, State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Faculty of Medicine and Life Sciences, Xiamen University, Xiamen, Fujian, China.ORCID 0000-0001-5066-4698
Xiangao JiangDepartment of Infectious Diseases, Wenzhou Central Hospital, The Dingli Clinical College of Wenzhou Medical University, Wenzhou, China.ORCID 0000-0002-8527-1015

Funding

NSFC-Zhejiang Joint Fund | Basic Public Welfare Research Program of Zhejiang Province LGF20H010003
6 · The paper itself

Abstract

Resistance to pyrazinamide (PZA) poses significant challenges to tuberculosis (TB) management, and prediction of susceptibility to PZA has been challenging. This study examined PZA resistance-associated gene mutations in 125 multidrug-resistant clinical isolates of IMPORTANCE: This study underscores the pressing need for reliable diagnostic methods to address high PZA resistance rates in TB cases, particularly in MDR-TB strains. By confirming that pncA mutations are the principal drivers of PZA resistance, we highlight the diagnostic potential of the MeltPro MTB/PZA assay as a rapid and effective alternative to conventional culture-based methods. Demonstrating sensitivity and specificity comparable to WGS and pDST, this assay offers a practical, accessible approach for timely PZA resistance prediction. It supports more tailored and effective MDR-TB treatment strategies, which are essential for optimizing patient care in both well-resourced and constrained settings.

Indexed as

Antitubercular AgentsDrug Resistance, Multiple, BacterialMycobacterium tuberculosisPyrazinamideTuberculosis, Multidrug-ResistantAmidohydrolasesBacterial ProteinsChinaHumansMicrobial Sensitivity TestsMutationWhole Genome SequencingAmidohydrolasesAntitubercular AgentsBacterial ProteinsPncA protein, Mycobacterium tuberculosisPyrazinamideMeltPro MTB/PZA assaymultidrug-resistant TBpDSTpncApyrazinamide

Identifiers

PMID40401978
PMCPMC12210976

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