ArticleMicrobiology spectrum2025
A practical protocol for correlative confocal fluorescence and transmission electron microscopy characterization of extracellular vesicles.
Article in Microbiology spectrum, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
4 citing papers in PubMed.
- No Dye Fits All: Illuminating the Challenges of Fluorescent Extracellular Vesicle Labeling.Journal of extracellular vesicles · 2026Article
- Environmental Regulation, Molecular Profiling, and Preliminary Functional Evaluation of Extracellular Vesicles fromFoods (Basel, Switzerland) · 2026Article
- An Optimized Positive Staining Protocol Method for Clear Visualization of Extracellular Vesicles by TEM Using Uranyless and Lead Citrate.Langmuir : the ACS journal of surfaces and colloids · 2026Article
- Extracellular Vesicle Release from Immune Cells in Cutaneous Leishmaniasis: Modulation byPathogens (Basel, Switzerland) · 2025Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Extracellular vesicles (EVs) have gained considerable attention in fungal biology research. However, imaging these membrane-bound particles presents challenges due to their nanoscopic size (typically <200 nm), which exceeds the resolution limit of conventional diffraction-limited laser scanning confocal microscopy (LSCM). While high-resolution techniques like transmission electron microscopy (TEM) offer superior spatial resolution, they are time-consuming, require specialized expertise, and are prone to artifacts that can interfere with accurate results. In this study, we propose a rapid method for confirming the vesicular nature of EVs using a correlative light and electron microscopy (CLEM) approach. EVs were isolated from culture filtrates of the model filamentous fungus
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Registered trials
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