Evidence map›Paper›PMID 40401935›Full record

ArticleMicrobiology spectrum2025

Multiplex amplicon sequencing for the comprehensive genotyping of

Hiroaki Kubota, Rumi Okuno, Tsuyoshi Kenri, Yumi Uchitani, Tsukasa Ariyoshi, Isao Yoshida, Kai Kobayashi, Morika Mitobe, Jun Suzuki, Kenji Sadamasu

Abstract read
In one paragraph

Article in Microbiology spectrum, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Immune dysregulation inFrontiers in immunology · 2026
    Review
  3. Article
  4. Genomic epidemiological analysis reveals new insights into the resurgence ofFrontiers in cellular and infection microbiology · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Hiroaki KubotaDepartment of Microbiology, Tokyo Metropolitan Institute of Public Health, Tokyo, Japan.ORCID 0000-0002-6339-8568
Rumi OkunoDepartment of Microbiology, Tokyo Metropolitan Institute of Public Health, Tokyo, Japan.
Tsuyoshi KenriDepartment of Bacteriology II, National Institute of Infectious Diseases, Tokyo, Japan.ORCID 0000-0002-3198-4263
Yumi UchitaniDepartment of Microbiology, Tokyo Metropolitan Institute of Public Health, Tokyo, Japan.ORCID 0009-0006-9694-6678
Tsukasa AriyoshiDepartment of Microbiology, Tokyo Metropolitan Institute of Public Health, Tokyo, Japan.ORCID 0000-0002-9294-5065
Isao YoshidaDepartment of Microbiology, Tokyo Metropolitan Institute of Public Health, Tokyo, Japan.ORCID 0009-0005-8330-8522
Kai KobayashiDepartment of Microbiology, Tokyo Metropolitan Institute of Public Health, Tokyo, Japan.ORCID 0000-0003-4524-284X
Morika MitobeDepartment of Microbiology, Tokyo Metropolitan Institute of Public Health, Tokyo, Japan.ORCID 0000-0002-1466-7661
Jun SuzukiDepartment of Microbiology, Tokyo Metropolitan Institute of Public Health, Tokyo, Japan.ORCID 0009-0006-2322-9176
Kenji SadamasuDepartment of Microbiology, Tokyo Metropolitan Institute of Public Health, Tokyo, Japan.ORCID 0000-0003-0541-706X

Funding

Japan Society for the Promotion of Science 22K07063Japan Society for the Promotion of Science 24K10230Ministry of Health, Labour and Welfare 24LA2003Takeda Science Foundation
6 · The paper itself

Abstract

Major genotyping methods used to characterize IMPORTANCE: Genotyping plays a central role in the molecular epidemiology of pathogenic bacteria, and many methods have been developed to identify prevalent lineages, infection routes, and antimicrobial resistance. Whole-genome sequencing generally provides most of the genetic information targeted by classic PCR-based schemes and has contributed to the construction of a simplified workflow for many bacterial species. However, several issues concerning the

Indexed as

Genotyping TechniquesHigh-Throughput Nucleotide SequencingMultiplex Polymerase Chain ReactionMycoplasma pneumoniaeDNA, BacterialDrug Resistance, BacterialGenome, BacterialGenotypeHumansMolecular EpidemiologyMultilocus Sequence TypingPneumonia, MycoplasmaPolymorphism, Single NucleotideRNA, Ribosomal, 23SWhole Genome SequencingWorkflowDNA, BacterialRNA, Ribosomal, 23Santibiotic resistancegenome analysisgenotypic identificationmolecular epidemiologyMycoplasma pneumoniaephylogenetic analysis

Identifiers

PMID40401935
PMCPMC12210885

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.