From lab reagent to metabolite: the riboswitch ligand guanidine as a relevant compound in bacterial physiology.

Efforts of the last 20 years in validating novel riboswitches led to the identification of numerous new motifs recognizing compounds with well-established biological functions. However, the recent characterization of widespread classes of riboswitches binding the nitrogen-rich compound guanidine raised questions regarding its physiological significance that has so far remained elusive. Recent findings established that certain bacterial species assimilate guanidine as a nitrogen source via guanidine-specific enzymes and transporters and that complete ammonium oxidizers can use it as a sole source of energy, reductant, and nitrogen. The frequent association of guanidine riboswitches with genes encoding guanidine efflux transporters also hints that bacteria may experience the burden of guanidine as a stressor during their lifestyle. A major gap in understanding the biology of guanidine resides in its natural source. While metabolic pathways responsible for guanidine synthesis were defined in plants, only a few guanidine-producing enzymes have been identified in bacteria, despite indications that the model organism