Evidence map›Paper›PMID 40401521›Full record

ArticleJCI insight2025

DAZAP1 maintains gastric cancer stemness by inducing mitophagy.

Peiling Zhang, Wei Wang, Hong Xiang, Yun Zhou, Qian Peng, Guolong Liu, Zhi-Xiang Xu, Lin Lu

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PubMed Full text at PMC Publisher
In one paragraph

Article in JCI insight, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Peiling ZhangDepartment of Medical Oncology, Guangzhou First People's Hospital, South China University of Technology, Guangzhou, China.
Wei WangDepartment of Gastric Surgery, Sun Yat-sen University Cancer Center, Guangzhou, Guangdong, China.
Hong XiangDepartment of Medical Oncology, Guangzhou First People's Hospital, South China University of Technology, Guangzhou, China.
Yun ZhouDepartment of Medical Oncology, Guangzhou First People's Hospital, South China University of Technology, Guangzhou, China.
Qian PengDepartment of Medical Oncology, Guangzhou First People's Hospital, South China University of Technology, Guangzhou, China.
Guolong LiuDepartment of Medical Oncology, Guangzhou First People's Hospital, South China University of Technology, Guangzhou, China.
Zhi-Xiang XuSchool of Life Sciences, Henan University, Kaifeng, Henan Province, China.
Lin LuDepartment of Medical Oncology, Guangzhou First People's Hospital, South China University of Technology, Guangzhou, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Stem cells play a pivotal role in the malignant behavior of gastric cancer (GC), complicating its treatment and prognosis. However, the regulatory mechanisms of GC stem cells (GCSCs) remain poorly understood. DAZ-associated protein 1 (DAZAP1), a splicing regulator linked to various malignancies, has an unclear role in GC. This study investigated DAZAP1's impact on GC stemness and its mechanisms. DAZAP1 promoted tumor progression in GCSCs, as shown by sphere formation assays and stemness marker analysis. Functional enrichment analysis suggested that DAZAP1 enhanced tumor stemness by promoting oxidative phosphorylation (OXPHOS), which was validated through Seahorse assays and measurements of mitochondrial potential. Transmission electron microscopy and immunofluorescence analyses demonstrated that DAZAP1 promoted mitophagy. RNA immunoprecipitation and PCR analysis revealed that DAZAP1 regulated the splicing and expression of the mitophagy-related gene ULK1 through nonsense-mediated mRNA decay. Rescue experiments showed that overexpression of ULK1 reversed the suppression of GC stemness and OXPHOS levels induced by DAZAP1 silencing. Our findings indicate that DAZAP1 reduces ULK1 decay, thereby activating mitophagy and enhancing OXPHOS to fulfill the metabolic demands of cancer stem cells. These findings highlight the therapeutic potential of DAZAP1 as a target for treating GC.

Indexed as

Autophagy-Related Protein-1 HomologMitophagyNeoplastic Stem CellsRNA-Binding ProteinsStomach NeoplasmsAlternative SplicingAnimalsCell LineGene Expression Regulation, NeoplasticGene Knockdown TechniquesHumansIntracellular Signaling Peptides and ProteinsKaplan-Meier EstimateMaleMiceMice, Inbred BALB CAutophagy-Related Protein-1 HomologDAZAP1 protein, humanDazap1 protein, mouseIntracellular Signaling Peptides and ProteinsRNA-Binding ProteinsULK1 protein, humanUlk1 protein, mouseAutophagyCell biologyGastric cancerMitochondriaOncologyStem cells

Identifiers

PMID40401521
PMCPMC12128983

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.