ArticleThe New phytologist2025
Role of LEAFLESS, an AP2/ERF family transcription factor, in the regulation of trichome specialized metabolism.
Article in The New phytologist, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
3 citing papers in PubMed.
- A Novel AP2/ERF Transcription Factor Controls Trichome Initiation and Fruit Development in Cucumber.Plant biotechnology journal · 2026Article
- Functional diversification of WRINKLED3 integrates fatty acid metabolism with insecticidal acylsugar production in Solanaceae species.Nature communications · 2026Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Acylsugars, specialized metabolites produced by trichomes of many solanaceous species, provide protection against biotic and abiotic stresses. Many acylsugar metabolic enzymes have been identified; however, regulatory factors remain unknown. Our multidisciplinary approaches identified LEAFLESS (APETALA 2/ ETHYLENE RESPONSE FACTOR (AP2/ERF) family member) as a positive regulator of acylsugar biosynthesis. Virus-induced gene silencing (VIGS) of LEAFLESS in Solanum pennellii (SpLFS/Sopen05g008450) revealed its distinct roles in two related but separate processes: acylsugar biosynthesis and trichome development. Most acylsugar (and several flavonoid) metabolic genes were downregulated in SpLFS-silenced plants and showed strong co-expression with SpLFS. Phylogenetic and additional data analyses indicated trichome-enriched expression of SpLFS orthologs in other acylsugar-producing solanaceous species, and VIGS of SpLFS orthologs in Nicotiana benthamiana reduced acylsugar production. Transcriptional reporter showed expression of SpLFS in type I/IV trichome tip cells, the site of acylsugar biosynthesis. Electrophoretic mobility shift assays indicated that SpLFS directly binds to promoters of several acylsugar (and flavonoid) metabolic genes. Additionally, data mining suggested remarkable spatiotemporal functional diversity: from coordinating leaf initiation at incipient primordia (previously reported for the S. lycopersicum ortholog SlLFS/Solyc05g013540) to regulating trichome specialized metabolism (acylsugar and flavonoid). Our work highlights a critical role of LEAFLESS in trichome specialized metabolism, paving the way to disentangle the acylsugar regulatory network.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.