Evidence map›Paper›PMID 40399284›Full record

ArticleNature communications2025

Deciphering signaling mechanisms and developmental dynamics in extraembryonic mesoderm specification from hESCs.

Baohua Niu, Da Wang, Yingjie Hu, Yundi Wang, Gaohui Shi, Zhongying Chen, Lifeng Xiang, Chi Zhang, Xuesong Wei, Ruize Kong and 5 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Baohua Niu *State Key Laboratory of Primate Biomedical Research; Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan, China.
Da Wang *State Key Laboratory of Primate Biomedical Research; Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan, China.ORCID http://orcid.org/0009-0007-2950-7701
Yingjie Hu *State Key Laboratory of Primate Biomedical Research; Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan, China.
Yundi Wang *State Key Laboratory of Primate Biomedical Research; Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan, China.
Gaohui Shi *State Key Laboratory of Primate Biomedical Research; Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan, China.ORCID http://orcid.org/0009-0003-1306-1136
Zhongying ChenUltrasound Department, The Third People's Hospital of Yunnan Province, Kunming, Yunnan, China.
Lifeng XiangDepartment of Reproductive Medicine, The First People's Hospital of Yunnan Province, Kunming, Yunnan, China.
Chi ZhangState Key Laboratory of Primate Biomedical Research; Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan, China.
Xuesong WeiState Key Laboratory of Primate Biomedical Research; Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan, China.
Ruize KongDepartment of Reproductive Medicine, The First People's Hospital of Yunnan Province, Kunming, Yunnan, China.
Hongzhi CaiState Key Laboratory of Primate Biomedical Research; Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan, China.
Weizhi JiState Key Laboratory of Primate Biomedical Research; Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan, China. wji@lpbr.cn.ORCID http://orcid.org/0000-0003-2550-4224
Yu YinState Key Laboratory of Primate Biomedical Research; Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan, China. yiny@lpbr.cn.ORCID http://orcid.org/0000-0001-6040-6115
Tianqing LiState Key Laboratory of Primate Biomedical Research; Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan, China. litq@lpbr.cn.
Zongyong AiState Key Laboratory of Primate Biomedical Research; Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan, China. aizy@lpbr.cn.ORCID http://orcid.org/0000-0003-1912-5690

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Extraembryonic mesoderm (ExM) is crucial for human development, yet its specification is poorly understood. Human embryonic stem cell (hESC)-based models, including embryoids and differentiated derivatives, are emerging as promising tools for studying ExM development. Despite this, the signaling mechanisms and developmental dynamics that underlie ExM specification from hESCs remain challenging to study. Here, we report that the modulation of BMP, WNT, and Nodal signaling pathways can rapidly (4-5 days) and efficiently ( ~90%) induce the differentiation of both naive and primed hESCs into ExM-like cells (ExMs). We reveal that ExM specification from hESCs predominantly proceeds through intermediates exhibiting a primitive streak (PS)-like gene expression pattern and delineate the regulatory roles of WNT and Nodal signaling in this process. Furthermore, we find that the initial pluripotent state governs hESC-based ExM specification by influencing signal response, cellular composition, developmental progression, and transcriptional characteristics of the resulting ExMs. Our study provides promising models for dissecting human ExM development and sheds light on the signaling principles, developmental dynamics, and influences of pluripotency states underlying ExM specification from hESCs.

Indexed as

Human Embryonic Stem CellsMesodermSignal TransductionBone Morphogenetic ProteinsCell DifferentiationCell LineGene Expression Regulation, DevelopmentalHumansNodal ProteinPrimitive StreakWnt Signaling PathwayBone Morphogenetic ProteinsNodal ProteinNODAL protein, human

Identifiers

PMID40399284
PMCPMC12095623

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.