Evidence map›Paper›PMID 40397572›Full record

ArticleCell reports2025

ADP-ribosylation of NuMA promotes DNA single-strand break repair and transcription.

Arwa A Abugable, Chunyan Liao, Sarah Antar, Matthew Dowson, Sherif F El-Khamisy

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Article in Cell reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Arwa A AbugableSchool of Biosciences, Firth Court, University of Sheffield, Sheffield, UK; The Healthy Lifespan and Neuroscience Institutes, University of Sheffield, Sheffield, UK.
Chunyan LiaoSchool of Biosciences, Firth Court, University of Sheffield, Sheffield, UK; The Healthy Lifespan and Neuroscience Institutes, University of Sheffield, Sheffield, UK.
Sarah AntarSchool of Biosciences, Firth Court, University of Sheffield, Sheffield, UK; The Healthy Lifespan and Neuroscience Institutes, University of Sheffield, Sheffield, UK; Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt.
Matthew DowsonSchool of Biosciences, Firth Court, University of Sheffield, Sheffield, UK; The Healthy Lifespan and Neuroscience Institutes, University of Sheffield, Sheffield, UK.
Sherif F El-KhamisySchool of Biosciences, Firth Court, University of Sheffield, Sheffield, UK; The Healthy Lifespan and Neuroscience Institutes, University of Sheffield, Sheffield, UK; Institute of Cancer Therapeutics, School of Pharmacy and Medical Sciences, University of Bradford, Bradford, UK. Electronic address: s.el-khamisy@bradford.ac.uk.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Single-strand breaks (SSBs) are prevalent DNA lesions implicated in genome instability. The nuclear mitotic apparatus protein (NuMA) has been reported to promote SSB repair (SSBR) and regulate transcription following oxidative stress. ADP-ribosylation, an important post-translational modification, regulates several processes, including chromatin remodeling, transcription, and DNA repair. To investigate its role in NuMA-dependent functions, we generated an ADP-ribosylation-deficient NuMA construct and report that NuMA ADP-ribosylation is required for its interaction with tyrosyl DNA phosphodiesterase 1 (TDP1), an SSBR player. Cells expressing ADP-ribosylation-deficient NuMA exhibit delayed SSBR kinetics following oxidative stress and reduced repair at promoter and enhancer regions, consistent with a role of NuMA in protecting non-coding regulatory regions from DNA damage. Furthermore, the expression of NuMA-regulated genes following oxidative stress requires ADP-ribosylation. Our findings demonstrate that ADP-ribosylation of NuMA promotes SSBR and transcription following oxidative stress, underscoring the importance of ADP-ribosylation in modulating DNA repair and gene expression.

Indexed as

ADP-RibosylationAntigens, NuclearCell Cycle ProteinsDNA Breaks, Single-StrandedDNA RepairNuclear Matrix-Associated ProteinsTranscription, GeneticHeLa CellsHumansOxidative StressPhosphoric Diester HydrolasesAntigens, NuclearCell Cycle ProteinsNuclear Matrix-Associated ProteinsNUMA1 protein, humanPhosphoric Diester HydrolasesTDP1 protein, humanADP-ribosylationbrain healthcancerCP: Molecular biologyDDRdementiaDNA damage responseDNA repairgene regulatory elementsIEGsimmediate early genesNuMAoxidative DNA damageoxidative stresssingle-strand DNA breakstranscription

Identifiers

PMID40397572
PMCPMC12187637

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.