Evidence map›Paper›PMID 40397283›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Generation of Human Naïve Pluripotent Stem Cell-Derived Blastoids in Thermoformed Microwell Platforms.

Dorian G Luijkx, Erik J Vrij, Ge Guo, Stefan Giselbrecht

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Dorian G LuijkxMERLN Institute of Technology-Inspired Regenerative Medicine, Department for Cell Biology-Inspired Tissue Engineering, Maastricht University, Maastricht, The Netherlands.
Erik J VrijMERLN Institute of Technology-Inspired Regenerative Medicine, Department for Cell Biology-Inspired Tissue Engineering, Maastricht University, Maastricht, The Netherlands.
Ge GuoLiving Systems Institute, University of Exeter, Exeter, UK.
Stefan GiselbrechtMERLN Institute of Technology-Inspired Regenerative Medicine, Department for Cell Biology-Inspired Tissue Engineering, Maastricht University, Maastricht, The Netherlands. s.giselbrecht@maastrichtuniversity.nl.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Due to the inaccessibility of early human embryos for large, robust studies, many questions regarding the mechanisms of early embryogenesis remain. To address these questions, multiple research groups have developed human stem cell-based models of the pre-implantation blastocyst stage. These models, known as blastoids, mimic several key processes that natural blastocysts undergo as they prepare to implant into the uterine wall. One of the main advantages of blastoids is their scalability, making them suitable for both screenings and molecular studies. To leverage this advantage, we established a protocol for the parallel formation and culture of blastoids in thermoformed microwell arrays. Thin-walled thermoformed microwell platforms allow for uniform, large-scale generation of blastoids and enable in situ high-resolution imaging through both widefield and confocal microscopy. Here we present a step-by-step protocol for the culture of blastoids in thermoformed microwell platforms.

Indexed as

BlastocystCell Culture TechniquesPluripotent Stem CellsEmbryonic DevelopmentHumansBlastocystsBlastoidsEmbryo modelsNaïve pluripotent stem cellsStem cell-based embryo modelsThermoformed microwells

Identifiers

PMID40397283

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.