Application of CRISPR-Based Epigenome Editing Tools for Engineering Programmable Embryo Models.

Gerrald A Lodewijk, Sayaka Kozuki, Carly Guiltinan, Benjamin R Topacio, S Ali Shariati

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Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

5 authors.

Gerrald A Lodewijk *Department of Biomolecular Engineering, University of California, Santa Cruz, CA, USA.

Sayaka Kozuki *Department of Biomolecular Engineering, University of California, Santa Cruz, CA, USA.

Carly GuiltinanDepartment of Biomolecular Engineering, University of California, Santa Cruz, CA, USA.

Benjamin R TopacioDepartment of Biomolecular Engineering, University of California, Santa Cruz, CA, USA.

S Ali Shariati *Department of Biomolecular Engineering, University of California, Santa Cruz, CA, USA. alish@ucsc.edu.

Funding

Molecular feedback between cell division cycle and differentiation in pluripotent stem cellsR35GM147395 · NIGMS · UNIVERSITY OF CALIFORNIA SANTA CRUZ · PI Ali Shariati · 2022 to 2026

$1.9M

NIGMS NIH HHS R35 GM147395

6 · The paper itself

Abstract

Stem cell-based embryo models (SEMs) have the potential to transform our understanding of early human embryogenesis. A critical step in engineering SEMs is the generation of the major cell types that compose preimplantation embryos including two primary extraembryonic lineages: (i) trophoblast cells, which are crucial for implantation and the establishment of maternal-fetal exchange, and (ii) hypoblast cells, which contribute to yolk sac formation. In addition, both cell types provide key signaling cues necessary for embryonic development. CRISPR-based epigenome editors are programmable devices that allow for efficient and precise activation (CRISPRa) or repression (CRISPRi) of cell fate-determining factors by modulating endogenous regulatory elements. Here, we present a step-by-step method to implement CRISPRa for controlling cell fate in embryonic stem cells based on our work in generation of CRISPR-programmed mouse embryo models.

Indexed as

CRISPR-Cas SystemsEmbryo, MammalianEmbryonic DevelopmentEpigenome EditingAnimalsEmbryonic Stem CellsHumansMiceCRISPR activationEmbryoidsEmbryo modelsEpigenomeMammalian embryoPluripotent stem cells

Identifiers

PMID40397277

DOI10.1046/j.1432-1033.2003.03694.x

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