Evidence map›Paper›PMID 40397106›Full record

ArticleArchives of toxicology2025

Molecular dosimetry of estragole and 1'-hydroxyestragole-induced DNA adduct formation, clastogenicity and cytotoxicity in human liver cell models.

G Ackermann, M Peil, C Quarz, A Schmidt, M Halaczkiewicz, A D Thomas, S Stegmüller, E Richling, G Manolikakes, M Christmann and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Archives of toxicology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

G AckermannDivision of Food Chemistry and Toxicology, Department of Chemistry, RPTU Kaiserslautern-Landau, Erwin-Schrödinger-Str. 52, 67663, Kaiserslautern, Germany.
M PeilDivision of Food Chemistry and Toxicology, Department of Chemistry, RPTU Kaiserslautern-Landau, Erwin-Schrödinger-Str. 52, 67663, Kaiserslautern, Germany.
C QuarzDivision of Food Chemistry and Toxicology, Department of Chemistry, RPTU Kaiserslautern-Landau, Erwin-Schrödinger-Str. 52, 67663, Kaiserslautern, Germany.
A SchmidtInstitute of Toxicology, University Medical Center, Obere Zahlbacher Str. 67, 55131, Mainz, Germany.
M HalaczkiewiczDivision of Organic Chemistry, Department of Chemistry, RPTU Kaiserslautern-Landau, Erwin-Schrödinger-Str. 54, Kaiserslautern, Germany.
A D ThomasSchool of Applied Sciences, University of the West of England, Bristol, BS16 1QY, UK.
S StegmüllerDivision of Food Chemistry and Toxicology, Department of Chemistry, RPTU Kaiserslautern-Landau, Erwin-Schrödinger-Str. 52, 67663, Kaiserslautern, Germany.
E RichlingDivision of Food Chemistry and Toxicology, Department of Chemistry, RPTU Kaiserslautern-Landau, Erwin-Schrödinger-Str. 52, 67663, Kaiserslautern, Germany.
G ManolikakesDivision of Organic Chemistry, Department of Chemistry, RPTU Kaiserslautern-Landau, Erwin-Schrödinger-Str. 54, Kaiserslautern, Germany.
M ChristmannInstitute of Toxicology, University Medical Center, Obere Zahlbacher Str. 67, 55131, Mainz, Germany.
J H KüpperDivision of Molecular Cell Biology, Faculty of Environment and Nature Science, Brandenburg University of Technology Cottbus-Senftenberg, Universitätsplatz 1, 01968, Senftenberg, Germany.
D SchrenkDivision of Food Chemistry and Toxicology, Department of Chemistry, RPTU Kaiserslautern-Landau, Erwin-Schrödinger-Str. 52, 67663, Kaiserslautern, Germany.
J FahrerDivision of Food Chemistry and Toxicology, Department of Chemistry, RPTU Kaiserslautern-Landau, Erwin-Schrödinger-Str. 52, 67663, Kaiserslautern, Germany. fahrer@chemie.uni-kl.de.ORCID 0000-0001-5225-2718

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The phenylpropene estragole (ES) is found in essential oils of herbs and spices, such as bitter fennel and basil. Humans are exposed to ES through the diet and phytomedicines. After its absorption, ES undergoes metabolic activation by CYP1A2 and SULT1A1 in the liver, which can give rise to DNA adducts and hepatocarcinogenesis. Until now, quantitative genotoxicity data for ES in human liver cells are scarce, correlating DNA adduct levels with critical effects such as clastogenicity. Here, we used human HepG2 and HepG2-CYP1A2 cells as well as primary human hepatocytes (PHH) to study the genotoxic, clastogenic and cytotoxic potential of ES and its crucial metabolite 1'-hydroxyestragole (1'OH-ES). In addition, primary rat hepatocytes (PRH) were used for selected endpoints. Treatment of HepG2-CYP1A2 cells with ES (0-2 mM) led to the concentration-dependent formation of E3'-N

Indexed as

AnisolesDNA AdductsHepatocytesMutagensAllylbenzene DerivativesAnimalsCell SurvivalCytochrome P-450 CYP1A2Dose-Response Relationship, DrugHep G2 CellsHistonesHumansMaleRats1'-hydroxyestragoleAllylbenzene DerivativesAnisolesCYP1A2 protein, humanCytochrome P-450 CYP1A2DNA AdductsestragoleH2AX protein, humanHistonesMutagensClastogenicityConcentration–response modellingCytotoxicityEstragoleGenotoxicityHuman hepatocytesLiver damagePhenylpropanoidsPlant toxin

Identifiers

PMID40397106
PMCPMC12408683

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.