Evidence map›Paper›PMID 40396977›Full record

ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2025

DNA Origami-Based CD44-Targeted Therapy Silences Stat3 Enhances Cartilage Regeneration and Alleviates Osteoarthritis Progression.

Qi Lv, Xiang Zhao, Songsong Teng, Xinmeng Jin, Ying Zhou, Yueyang Sun, Hao Pei, Zuoqin Yan, Chunhui Ma

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Review
  2. Article
  3. Review
  4. Article
  5. DNA-programmed cell assembly: from cells, tissues to organoids.Frontiers in bioengineering and biotechnology · 2025
    Review
  6. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Qi LvDepartment of Medical Imaging, Tongji Hospital, School of Medicine, Tongji University, Shanghai, 200065, China.
Xiang ZhaoDepartment of Surgery of Spine and Spinal Cord, Henan Provincial People's Hospital, Zhengzhou, 450003, China.
Songsong TengSchool of Chemistry and Life Sciences, Suzhou University of Science and Technology, Suzhou, 215009, China.
Xinmeng JinDepartment of Orthopedic Surgery, Shanghai General Hospital, Shanghai Jiao Tong University, Shanghai, 200080, China.
Ying ZhouShanghai Key laboratory of green chemistry and chemical Processes, School of chemistry and Molecular engineering, Shanghai center of Brain inspired intelligent Materials and devices, East China normal University, Shanghai, 200241, China.
Yueyang SunShanghai Key laboratory of green chemistry and chemical Processes, School of chemistry and Molecular engineering, Shanghai center of Brain inspired intelligent Materials and devices, East China normal University, Shanghai, 200241, China.
Hao PeiShanghai Key laboratory of green chemistry and chemical Processes, School of chemistry and Molecular engineering, Shanghai center of Brain inspired intelligent Materials and devices, East China normal University, Shanghai, 200241, China.ORCID 0000-0002-6885-6708
Zuoqin YanDepartment of Orthopedic Surgery, Shanghai Geriatric Medical Center, Shanghai, 201104, China.
Chunhui MaDepartment of Orthopedic Surgery, Shanghai Geriatric Medical Center, Shanghai, 201104, China.

Funding

Shanghai Natural Science Foundation 22ZR1450300Shanghai Natural Science Foundation 22ZR1456600
6 · The paper itself

Abstract

Osteoarthritis (OA) is a widespread musculoskeletal disorder affecting ≈600 million people globally, and small interfering RNA (siRNA) therapy shows potential in targeting OA progression. However, the efficient and targeted delivery of siRNA remains a major challenge due to issues with tissue specificity and degradation in vivo. In this study, A DNA origami-based chondrocyte-targeted delivery system (OCS) is designed for siRNA delivery to OA-affected cartilage. The DNA origami is engineered to load with siRNA targeting signal transducer and activator of transcription 3 (Stat3), a key regulator of inflammation and cartilage degradation, and is functionalized with anti-CD44 aptamers for selective targeting of OA chondrocytes. In vitro, the DNA origami system effectively delivers siRNA to diseased chondrocytes, silencing matrix metalloproteinases expression and reducing inflammation. In OA rat models, it preserves cartilage integrity, promotes regeneration, and mitigates ECM degradation without evident side effects. These findings highlight DNA origami as a promising platform for siRNA-based OA therapy, offering a promising solution to the challenges of targeted and efficient siRNA delivery.

Indexed as

DNAHyaluronan ReceptorsOsteoarthritisRegenerationSTAT3 Transcription FactorAnimalsCartilageCartilage, ArticularChondrocytesDisease Models, AnimalDisease ProgressionHumansMaleRatsRats, Sprague-DawleyRNA, Small InterferingDNAHyaluronan ReceptorsRNA, Small InterferingSTAT3 Transcription FactorCD44 targetingDNA triangle origamiosteoarthritissi‐Stat3

Identifiers

PMID40396977
PMCPMC12362795

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.