Evidence map›Paper›PMID 40395982›Full record

ArticleAutophagy reports2025

BECLIN-1 is essential for the maintenance of gastrointestinal epithelial integrity by regulating endocytic trafficking, F-actin organization, and lysosomal function.

Juliani Juliani, Sharon Tran, Tiffany J Harris, Peter De Cruz, Sarah L Ellis, Paul A Gleeson, John M Mariadason, Kinga Duszyc, Alpha S Yap, Erinna F Lee and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Autophagy reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
  3. The Biological Role and Clinical Significance of BECLIN-1 in Cancer.International journal of molecular sciences · 2025
    Review
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Juliani JulianiCell Death and Survival Laboratory, Olivia Newton-John Cancer Research Institute, Heidelberg, Australia.ORCID https://orcid.org/0009-0006-5681-1197
Sharon TranCell Death and Survival Laboratory, Olivia Newton-John Cancer Research Institute, Heidelberg, Australia.
Tiffany J HarrisCell Death and Survival Laboratory, Olivia Newton-John Cancer Research Institute, Heidelberg, Australia.
Peter De CruzDepartment of Gastroenterology, Austin Health, Melbourne, Australia.
Sarah L EllisCell Death and Survival Laboratory, Olivia Newton-John Cancer Research Institute, Heidelberg, Australia.
Paul A GleesonDepartment of Biochemistry and Pharmacology and Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Melbourne, Australia.
John M MariadasonCell Death and Survival Laboratory, Olivia Newton-John Cancer Research Institute, Heidelberg, Australia.
Kinga DuszycInstitute for Molecular Bioscience, The University of Queensland, Brisbane, Australia.
Alpha S YapInstitute for Molecular Bioscience, The University of Queensland, Brisbane, Australia.
Erinna F LeeCell Death and Survival Laboratory, Olivia Newton-John Cancer Research Institute, Heidelberg, Australia.ORCID https://orcid.org/0000-0003-1255-9808
Walter D FairlieCell Death and Survival Laboratory, Olivia Newton-John Cancer Research Institute, Heidelberg, Australia.ORCID https://orcid.org/0000-0002-2498-1160

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Disrupted intestinal homeostasis and barrier function contribute to the development of diseases such as inflammatory bowel disease. BECLIN-1, a core component of two class III phosphatidylinositol 3 kinase complexes, has a dual role in autophagy and endocytic trafficking. Emerging evidence suggests that its endocytic trafficking function is essential for intestinal integrity. To investigate the fatal gastrointestinal phenotype observed in BECLIN-1 knockout mice, we used organoids derived from these animals to show that BECLIN-1 deletion disrupts the localization of CADHERIN1/ECADHERIN to adherens junctions and OCCLUDIN to tight junctions. Impaired cargo trafficking to the lysosome was also observed. Filamentous actin cytoskeleton also became disorganized though there were no changes in its spatial interaction with CATENIN BETA1/BETA-CATENIN nor in BETA-CATENIN localization. The trafficking defects were all less pronounced or absent in organoids lacking an autophagy-only regulator, ATG7, emphasizing BECLIN-1's trafficking role in maintaining gut homeostasis and barrier function. These findings advance our understanding of epithelial dysfunction and the mechanisms underlying intestinal diseases.

Indexed as

ATG7autophagyBECLIN-1cytoskeletonE-CADHERINendocytosisF-actinIBDintestinal homeostasislysosomeOCCLUDIN

Identifiers

PMID40395982
PMCPMC11980461

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.