Evidence map›Paper›PMID 40395657›Full record

ArticleRevista panamericana de salud publica = Pan American journal of public health2025

Genetic screening of FFPE breast cancer biopsies for the

Sheherazade Crystal Abrahim, Dulari Bansraj, Royanne Edwards, Reinand Thompson, Roma Rambaran, Allana Roach, Wayne A Warner, A V Chalapathi Rao, Chandrashekar Unakal, Rajini Rani Haraksingh

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Article in Revista panamericana de salud publica = Pan American journal of public health, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

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5 · Who and what money

Authors and funding

10 authors.

Sheherazade Crystal AbrahimDepartment of Life Sciences Faculty of Science and Technology The University of the West Indies St. Augustine Trinidad and Tobago Department of Life Sciences, Faculty of Science and Technology, The University of the West Indies, St. Augustine, Trinidad and Tobago.
Dulari BansrajDepartment of Life Sciences Faculty of Science and Technology The University of the West Indies St. Augustine Trinidad and Tobago Department of Life Sciences, Faculty of Science and Technology, The University of the West Indies, St. Augustine, Trinidad and Tobago.
Royanne EdwardsDepartment of Life Sciences Faculty of Science and Technology The University of the West Indies St. Augustine Trinidad and Tobago Department of Life Sciences, Faculty of Science and Technology, The University of the West Indies, St. Augustine, Trinidad and Tobago.
Reinand ThompsonDepartment of Para-Clinical Sciences Faculty of Medical Sciences The University of the West Indies St. Augustine Trinidad and Tobago Department of Para-Clinical Sciences, Faculty of Medical Sciences, The University of the West Indies, St. Augustine, Trinidad and Tobago.
Roma RambaranDepartment of Para-Clinical Sciences Faculty of Medical Sciences The University of the West Indies St. Augustine Trinidad and Tobago Department of Para-Clinical Sciences, Faculty of Medical Sciences, The University of the West Indies, St. Augustine, Trinidad and Tobago.
Allana RoachDepartment of Para-Clinical Sciences Faculty of Medical Sciences The University of the West Indies St. Augustine Trinidad and Tobago Department of Para-Clinical Sciences, Faculty of Medical Sciences, The University of the West Indies, St. Augustine, Trinidad and Tobago.
Wayne A WarnerOncology Division Washington University School of Medicine St. Louis United States of America Oncology Division, Washington University School of Medicine, St. Louis, United States of America.
A V Chalapathi RaoDepartment of Para-Clinical Sciences Faculty of Medical Sciences The University of the West Indies St. Augustine Trinidad and Tobago Department of Para-Clinical Sciences, Faculty of Medical Sciences, The University of the West Indies, St. Augustine, Trinidad and Tobago.
Chandrashekar UnakalDepartment of Para-Clinical Sciences Faculty of Medical Sciences The University of the West Indies St. Augustine Trinidad and Tobago Department of Para-Clinical Sciences, Faculty of Medical Sciences, The University of the West Indies, St. Augustine, Trinidad and Tobago.
Rajini Rani HaraksinghDepartment of Life Sciences Faculty of Science and Technology The University of the West Indies St. Augustine Trinidad and Tobago Department of Life Sciences, Faculty of Science and Technology, The University of the West Indies, St. Augustine, Trinidad and Tobago.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Objective: To investigate whether the quality and quantity of genomic DNA harnessed from existing formalin-fixed paraffin-embedded (FFPE) breast cancer biopsy tissue samples in the public health system of Trinidad and Tobago (T&T) were sufficient for downstream genetic testing and to investigate the occurrence of the common breast cancer susceptibility gene 1 ( Methods: Genomic DNA was extracted from 67 FFPE samples using a standard protocol (Qiagen). Samples were genotyped using polymerase chain reaction (PCR) and Sanger sequencing. Results: The genomic DNA was highly fragmented in the 250-500 bp range. The quality and quantity only allowed testing of one variant. This study successfully genotyped 34 of 67 FFPE breast cancer tissue biopsy samples for the Conclusion: Existing FFPE cancer tissue biopsies in the public health system in T&T are of limited utility for genetic testing. The absence of the

Indexed as

Breast neoplasmsgenes, BRCA1genetic testingTrinidad and Tobago

Identifiers

PMID40395657
PMCPMC12091074

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.