Evidence map›Paper›PMID 40394651›Full record

ArticleEuropean journal of medical research2025

Growth and size of stem cells from human exfoliated deciduous teeth cultured in platelet lysate vs. fetal bovine serum for regenerative applications.

Ardeshir Talebi, Sanaz Razi, Sahar Talebi, Nosrat Nourbakhsh, Hossein Talebi, Sareh Soroushzadeh, Samin Razi, Seyed Ali Mosaddad

Abstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in European journal of medical research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

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5 · Who and what money

Authors and funding

8 authors.

Ardeshir TalebiDepartment of Pathology, Medical School, Dental Research Center, Dental Research Institute, Isfahan University of Medical Sciences, Isfahan, Iran.
Sanaz RaziDepartment of Pathology, Medical School, Dental Research Center, Dental Research Institute, Isfahan University of Medical Sciences, Isfahan, Iran.
Sahar TalebiSchool of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran. sahar.talebi.dds@gmail.com.
Nosrat NourbakhshDepartment of Pediatric Dentistry, Dental Research Center, Dental Research Institute, School of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran.
Hossein TalebiDepartment of Cellular and Molecular Biology, Falavarjan Islamic Azad University, Isfahan, Iran.
Sareh SoroushzadehAcademic Center for Education, Culture and Research, Isfahan, Iran.
Samin RaziSchool of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran.
Seyed Ali MosaddadDepartment of Research Analytics, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, India.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundStem cells from human exfoliated deciduous teeth (SHED) can differentiate into functioning neurons and oligodendrocytes and exhibit immunomodulatory and regenerative properties. This study aimed to compare the growth and size of SHED cultured in human platelet lysate (hPL) and fetal bovine serum (FBS), with the goal of evaluating their potential use in regenerative medicine.

methodsHealthy deciduous teeth with uninflamed pulp were used for culture. Growth, proliferation, and morphology of the cultured SHED, as well as the mean surface area, length, and width of the cells, were compared between FBS and hPL.

resultsThe mean surface area of the cultured SHED was lower on FBS than on hPL on days 4, 6, and 9 of passage 0, higher on FBS on day 2, and comparable on days 5 and 10. Comparison of growth, proliferation, and morphology of the cultured SHED on FBS and hPL on days 3 and 9 of passage 0 showed no significant difference. In addition, there was no significant difference regarding growth, proliferation, and morphology of the cells between FBS and hPL on the same days of passages 1, 2, 3, and 4. The total mean length of the cells was lower on hPL than on FBS within 10 days (137.9 vs. 148.1 µm), while the total mean width of the cells was higher on hPL than on FBS within 10 days (28 vs. 26 µm).

conclusionsFBS and hPL were comparable regarding growth, proliferation, and morphology of SHED, with higher mean width and lower mean length on hPL than on FBS within 10 days and overall comparable mean surface area after 10 days of passage 0. Therefore, FBS and hPL appear to be relatively similar for the culture of SHED, and hPL can replace FBS for this purpose.

Indexed as

Blood PlateletsStem CellsTooth, DeciduousAnimalsCattleCell Culture TechniquesCell DifferentiationCell ProliferationCells, CulturedChildCulture MediaHumansRegenerative MedicineCulture MediaFetal bovine serumHuman platelet lysateSHED

Identifiers

PMID40394651
PMCPMC12093836

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