Evidence map›Paper›PMID 40394619›Full record

Trial reportRespiratory research2025

Lung lipids associated with smoking and ECIG use in a cross-sectional study and clinical trial.

Joseph P McElroy, Min-Ae Song, John R Barr, Michael S Gardner, Garret Kinnebrew, Zsuzsanna Kuklenyik, Jennifer D Kusovschi, Jon C Rees, Benjamin C Blount, MuChun Tsai and 6 more

Registry-linked trialAbstract readRandomized Controlled Trial
PubMed Full text at PMC Publisher
In one paragraph

Trial report in Respiratory research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT02596685 (Effects of Electronic Cigarette Use on the Human Lung), which is not on this map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

NCT02596685 nacompletednot on this map

Effects of Electronic Cigarette Use on the Human Lung

TypeinterventionalSponsorOhio State University Comprehensive Cancer CenterRan2015 to 2021Enrolled142ConditionsCurrent Smoker, Never Smoker, Tobacco Use Disorder, Former SmokerArmsBronchoscopy, Electronic Cigarette, Laboratory Biomarker Analysis, Questionnaire Administration
3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

Joseph P McElroy *Center for Biostatistics, Department of Biomedical Informatics, College of Medicine, The Ohio State University, Columbus, OH, USA.
Min-Ae Song *Division of Environmental Health Sciences, College of Public Health, The Ohio State University, Columbus, OH, USA.
John R BarrDivision of Laboratory Sciences, Centers for Disease Control and Prevention, National Center for Environmental Health, Atlanta, GA, USA.
Michael S GardnerDivision of Laboratory Sciences, Centers for Disease Control and Prevention, National Center for Environmental Health, Atlanta, GA, USA.
Garret KinnebrewDepartment of Biomedical Informatics, College of Medicine, The Ohio State University, Columbus, OH, USA.
Zsuzsanna KuklenyikDivision of Laboratory Sciences, Centers for Disease Control and Prevention, National Center for Environmental Health, Atlanta, GA, USA.
Jennifer D KusovschiDivision of Laboratory Sciences, Centers for Disease Control and Prevention, National Center for Environmental Health, Atlanta, GA, USA.
Jon C ReesDivision of Laboratory Sciences, Centers for Disease Control and Prevention, National Center for Environmental Health, Atlanta, GA, USA.
Benjamin C BlountDivision of Laboratory Sciences, Centers for Disease Control and Prevention, National Center for Environmental Health, Atlanta, GA, USA.
MuChun TsaiComprehensive Cancer Center, The Ohio State University and James Cancer Hospital, 460 W. 10 th Avenue, 9 th Floor, Suite D920, Columbus, OH, 43210-1240, USA.
Mark D WewersComprehensive Cancer Center, The Ohio State University and James Cancer Hospital, 460 W. 10 th Avenue, 9 th Floor, Suite D920, Columbus, OH, 43210-1240, USA.
Sahar KamelComprehensive Cancer Center, The Ohio State University and James Cancer Hospital, 460 W. 10 th Avenue, 9 th Floor, Suite D920, Columbus, OH, 43210-1240, USA.
Sarah A ReisingerComprehensive Cancer Center, The Ohio State University and James Cancer Hospital, 460 W. 10 th Avenue, 9 th Floor, Suite D920, Columbus, OH, 43210-1240, USA.
Amarnath SinghComprehensive Cancer Center, The Ohio State University and James Cancer Hospital, 460 W. 10 th Avenue, 9 th Floor, Suite D920, Columbus, OH, 43210-1240, USA.
Daniel Y WengComprehensive Cancer Center, The Ohio State University and James Cancer Hospital, 460 W. 10 th Avenue, 9 th Floor, Suite D920, Columbus, OH, 43210-1240, USA.
Peter G ShieldsComprehensive Cancer Center, The Ohio State University and James Cancer Hospital, 460 W. 10 th Avenue, 9 th Floor, Suite D920, Columbus, OH, 43210-1240, USA. Peter.Shields@osumc.edu.

Funding

Translational Therapeutics Research Program (TT)P30CA016058 · NCI · OHIO STATE UNIVERSITY · PI Daniel G. Stover · 1985 to 2026
$132.3M
The Ohio State University Center for clinical and Translational ScienceUL1TR001070 · NCATS · OHIO STATE UNIVERSITY · PI JACKSON, REBECCA D · 2013 to 2017
$22.6M
Project 4: Comprehension of Health Risks in More and Less Arousing Affe p240-275P50CA180908 · NCI · OHIO STATE UNIVERSITY · PI SHIELDS, PETER G., WEWERS, MARY ELLEN · 2013 to 2017
$18.9M
Center for Tobacco Products P50CA180908NCATS NIH HHS UL1 TR001070NCATS NIH HHS UL1TR001070NCI NIH HHS P30 CA016058NCI NIH HHS P50 CA180908
6 · The paper itself

Abstract

backgroundWhile electronic cigarettes (ECIG) may have lower toxicant delivery than cigarettes, ECIG-liquids and aerosols still contain toxicants that can potentially disrupt lung lipid homeostasis.

methodsParticipants from two studies underwent bronchoscopy and bronchoalveolar lavage (BAL). Ninety-eight participants (21-44 years old) were included in a cross-sectional study, with 17 ECIG users, 52 non-smokers, and 29 smokers. In the four-week clinical trial, 30 non-smokers were randomly assigned to use nicotine-free, flavorless ECIG or no use. A panel of 75 quantifiable lipid species and 7 lipid classes were assessed in the BAL using two tandem mass spectrometry (MS/MS) platforms. Ten cytokines and lipid-laden macrophages (LLM) were analyzed using the V-PLEX Plus Proinflam Combo 10 panel and Oil Red O staining, respectively.

resultsIn the cross-sectional study, 43 lipids were associated with smoking status at FDR<0.1, including two between ECIG and non-smokers (PC(14:0/18:1) and PC(18:0/14:0)) in pairwise follow-up analyses (Bonferroni-adjusted p<0.017). Associations between lipid species and cotinine, inflammatory markers, including IL-1β and IL-8, and LLM were also identified, as well as differences in lipid classes between smokers and the other groups. Smokers had higher saturated lipids, including ceramide (CER), sphingomyelin (SM), and diacylglycerol (DAG) than that of non-smokers and ECIG users. No significant associations were identified in the 4-week clinical trial.

conclusionsSmoking was associated with altered lipid levels, as compared to both non-smokers and ECIG users; the majority were downregulated and ECIG effects tend to be smaller in magnitude than smoking effects, although some were different than those in the smokers group. This is a novel study of healthy individuals examining lipidomic differences between smokers, ECIG users, and non-smokers, indicating potential roles of smoking and ECIG-related lipid alterations in pulmonary disease.

trial registrationThe study was approved by The OSU Institutional Review Board (OSU-2015C0088) in accordance with its ethical standards, the Helsinki declaration, and the Belmont Report, and is registered on Clinicaltrials.gov (NCT02596685; 2015-11-04).

Indexed as

Electronic Nicotine Delivery SystemsLipid MetabolismLipidsLungSmokingVapingAdultBronchoalveolar Lavage FluidBronchoscopyCross-Sectional StudiesFemaleHumansMaleYoung AdultLipidsElectronic CigaretteLipidomicsSmoking

Identifiers

PMID40394619
PMCPMC12093903

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Registered trials

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.