Evidence map›Paper›PMID 40394206›Full record

ArticleScientific reports2025

Gut microbial ecology and function of a Pakistani cohort with Iron deficiency Anemia.

Hajra Khurshid, Muhammad Bilal Jamshaid, Zahra Salahuudin, Kashaf Sibtain, Iqra Fayyaz, Aqsa Ameer, Caroline Kerbiriou, Shona Mckirdy, Sobia Nawaz Malik, Konstantinos Gerasimidis and 5 more

Abstract read
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Nutrients · 2026
    Article
  3. HS-AssociatedMicroorganisms · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Hajra KhurshidDepartment of Biosciences, COMSATS University Islamabad, Islamabad, 45550, Pakistan.
Muhammad Bilal JamshaidDepartment of Biosciences, COMSATS University Islamabad, Islamabad, 45550, Pakistan.
Zahra SalahuudinDepartment of Biosciences, COMSATS University Islamabad, Islamabad, 45550, Pakistan.
Kashaf SibtainDepartment of Biosciences, COMSATS University Islamabad, Islamabad, 45550, Pakistan.
Iqra FayyazDepartment of Biosciences, COMSATS University Islamabad, Islamabad, 45550, Pakistan.
Aqsa AmeerDepartment of Biosciences, COMSATS University Islamabad, Islamabad, 45550, Pakistan.
Caroline KerbiriouSchool of Medicine, Dentistry & Nursing, Glasgow Royal Infirmary, Glasgow, G31 2ER, UK.
Shona MckirdySchool of Medicine, Dentistry & Nursing, Glasgow Royal Infirmary, Glasgow, G31 2ER, UK.
Sobia Nawaz MalikDepartment of Bioscience, Grand Asian University Sialkot, Sialkot, Pakistan.
Konstantinos GerasimidisSchool of Medicine, Dentistry & Nursing, Glasgow Royal Infirmary, Glasgow, G31 2ER, UK.
Zobia NoreenDepartment of Biosciences, COMSATS University Islamabad, Islamabad, 45550, Pakistan.
Sundus JavedDepartment of Biosciences, COMSATS University Islamabad, Islamabad, 45550, Pakistan.
Aafia TariqCosmesurge Hospital, Rawalpindi, Pakistan.
Umer Zeeshan IjazWater & Environment Research Group, Mazumdar-Shaw Advanced Research Centre, University of Glasgow, Glasgow, G11 6EW, UK. Umer.Ijaz@glasgow.ac.uk.
Aamira TariqDepartment of Biosciences, COMSATS University Islamabad, Islamabad, 45550, Pakistan. aamira_tariq@comsats.edu.pk.

Funding

Engineering and Physical Sciences Research Council EP/V030515/1Medical Research Council MR/Z50628X/1
6 · The paper itself

Abstract

Iron deficiency anemia (IDA) is a major public health concern among women of reproductive age leading to high maternal mortality. Pakistan being one of the Lower -Middle income countries (LMIC) is facing this challenge tremendously. The objective of this study is to determine the impact of IDA on gut microbial diversity as well as its relationship with microbial metabolites.16 S rRNA gene profiling of healthy and IDA affected meconium samples was performed with additional meta-data collected through questionnaire. The anemia was linked to different dietary parameters through chi-square test of independence and Generalized Linear Latent Variable Model (GLLVM). Anaerobic bacterial genera such as Coprococcus, Anaerovoracaceae, strongly associated with anemia and negatively correlated with red meat and fish consumption. Moreover, these microbes positively correlated with branched short chain fatty acids (BSCFAs) production. BSCFAs have strong implications in metabolic disorders. This study provides a snapshot of how anemia modulates gut microbial diversity and microbial metabolites production which may have an impact on iron metabolism.

Indexed as

Anemia, Iron-DeficiencyGastrointestinal MicrobiomeAdultBacteriaCohort StudiesDietFemaleHumansIronPakistanRNA, Ribosomal, 16SIronRNA, Ribosomal, 16SBranched short chain fatty acids (BSCFA)Iron deficiency anemia (IDA)Short chain fatty acids (SCFA)

Identifiers

PMID40394206
PMCPMC12092841

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.