miRNA-200a suppresses GNAI1 and PLCB4 to modulate skin pigmentation in cashmere goats.

Jianping Li, Baoyu Zhang, Runlai Liu, Xinyu Li, Xuezhao Sun, Qiaoling Zhang

Abstract read

In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

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3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

Polymorphic microsatellite loci from ddRAD sequencing data in different Indian yak populations adapted to diverse climatic conditions.BMC genomics · 2025
Article

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Jianping LiCollege of Animal Science and Technology, Jilin Agricultural Science and Technology University, Jilin, 132101, China.

Baoyu ZhangCollege of Veterinary Medicine, Jilin University, Changchun, 130062, China.

Runlai LiuCollege of Veterinary Medicine, Jilin University, Changchun, 130062, China.

Xinyu LiCollege of Veterinary Medicine, Jilin University, Changchun, 130062, China.

Xuezhao SunCollege of Animal Science and Technology, Jilin Agricultural Science and Technology University, Jilin, 132101, China. Xuezhao.Sun@jlnku.edu.cn.

Qiaoling ZhangCollege of Veterinary Medicine, Jilin University, Changchun, 130062, China. zql@jlu.edu.cn.

Funding

Education Department of Jilin Province JJKH20220399KJ

6 · The paper itself

Abstract

Coat colour formation in mammals is influenced by melanogenesis and pigmentation processes regulated by miRNAs, including miRNA-200a. Although miRNA-200a is differentially expressed in the skin of cashmere goats with varying coat colours, its regulatory mechanism remains unclear. In this study, miRNA-200a target genes were predicted using miRBase and TargetScan, identifying GNAI1 and PLCB4 as the target genes through GO and KEGG analyses. Dual-luciferase assays using wild-type and mutant plasmids confirmed a direct interaction between miRNA-200a and the 3'UTR regions of these genes. RT-qPCR and Western blot analyses demonstrated that the expression levels of miRNA-200a and its target genes differed significantly between black and white goat skin. In HaCaT cells, transfection with miRNA-200a mimics or inhibitors altered GNAI1 and PLCB4 expression at both mRNA and protein levels. To validate these findings in vivo, subcutaneous injection of antagomiR-200a into BALB/c mice significantly reduced melanin content (P < 0.01) and increased the expression of GNAI1 and PLCB4. These results indicate that miRNA-200a modulates skin pigmentation by suppressing GNAI1 and PLCB4, thereby influencing coat colour in cashmere goats. This study provides a foundational understanding for leveraging genetic regulation to enhance coat colour diversity and develop naturally pigmented breeds.

Indexed as

GoatsMicroRNAsPhospholipase C betaSkin Pigmentation3' Untranslated RegionsAnimalsGene Expression RegulationHumansMelaninsMiceMice, Inbred BALB C3' Untranslated RegionsMelaninsMicroRNAsPhospholipase C betaCashmere goatCoat colourGNAI1MelanogenesisMiRNA-200aPigmentationPLCB4

Identifiers

PMID40394057

DOI10.1038/s41598-025-01956-6

PMCPMC12092684

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LicenceCC BY-NC-ND

Read underepoch 390

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None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.