Evidence map›Paper›PMID 40394012›Full record

ArticleNature communications2025

A super versatile flexizyme system with phenol esters for genetic code reprogramming.

Yun-Kyu Choi, Takayuki Katoh, Adam Beattie, Hiroaki Suga

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Yun-Kyu ChoiDepartment of Chemistry, Graduate School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, Japan.ORCID http://orcid.org/0009-0007-5104-052X
Takayuki KatohDepartment of Chemistry, Graduate School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, Japan. katoh@chem.s.u-tokyo.ac.jp.ORCID http://orcid.org/0000-0001-6550-6203
Adam BeattieDepartment of Chemistry, Graduate School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, Japan.
Hiroaki SugaDepartment of Chemistry, Graduate School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, Japan. hsuga@chem.s.u-tokyo.ac.jp.ORCID http://orcid.org/0000-0002-5298-9186

Funding

Human Frontier Science Program (HFSP) RGP0015/2017MEXT | Japan Society for the Promotion of Science (JSPS) 22H00439MEXT | Japan Society for the Promotion of Science (JSPS) JP21H05618
6 · The paper itself

Abstract

Genetic code reprogramming enables ribosomal incorporation of multiple nonproteinogenic amino acids (npAAs), supporting bioactive peptide development. Flexizyme technology with npAA-benzyl(thio)esters (BZEs) as acyl-donors has been crucial for preparing diverse npAA-tRNAs. However, low acylation yields for some npAAs hinder peptide library construction. Here we report a versatile flexizyme system using phenol esters as alternative acyl-donors. Computational pK

Indexed as

Amino AcidsEstersGenetic CodePhenolPhenolsPeptide LibraryPeptidesRibosomesRNA, TransferTransfer RNA AminoacylationAmino AcidsEstersPeptide LibraryPeptidesPhenolPhenolsRNA, Transfer

Identifiers

PMID40394012
PMCPMC12092696

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.