ArticlePloS one2025
Rottlerin inhibits macropinocytosis of Porcine Reproductive and Respiratory Syndrome Virus through the PKCδ-Cofilin signaling pathway.
Article in PloS one, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed.
- The roles and mechanisms of natural compounds in preventing and treating viral reproductive disorders in major economic livestock.Virulence · 2026Review
- Marine medaka PKCα promotes red-spotted grouper nervous necrosis virus entry by orchestrating MYL3-mediated macropinocytosis and cofilin-dependent actin remodeling.Journal of virology · 2026Article
- AI-Based Respiratory Monitoring-Guided Evaluation of Rottlerin Therapy for PRRS in Grower-Finisher Pig Farms.Viruses · 2026Article
- Tuning probe permeabilityChemical science · 2025Article
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Authors and funding
5 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Rottlerin exerts antiviral activity against various enveloped viruses, yet the mechanism by which it inhibits viral replication and the associated signaling pathways remains unclear. Here, we investigated the mechanisms for the antiviral effects of rottlerin against Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) in vitro. We demonstrate that PRRSV enters host cells via macropinocytosis. Rottlerin, a PKCδ inhibitor, partially inhibits PRRSV entry by decreasing actin polymerization, as evidenced by alterations in actin dynamics. LIM domain kinase 1 (LIMK1) is essential for PRRSV replication, and PRRSV infection-induced cofilin activation, which was reversed by rottlerin treatment. Our findings suggest that a subset of PRRSV utilizes PKCδ-mediated actin dynamics to enter cells via macropinocytosis, and that rottlerin is a potential antiviral molecule targeting this entry pathway.
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