Evidence map›Paper›PMID 40392831›Full record

ArticlePloS one2025

Comparative genomic analysis of Streptococcus suis sequence type 105 and development of a PCR diagnostic tool.

Parichart Boueroy, Peechanika Chopjitt, Jednipit Borthong, Thidathip Wongsurawat, Piroon Jenjaroenpun, Rapeephan Duangjanchot, Bhanubong Saiboonjan, Rujirat Hatrongjit, Anusak Kerdsin

Abstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Parichart BoueroyFaculty of Public Health, Kasetsart University Chalermphrakiat Sakon Nakhon Province Campus, Sakon Nakhon, Thailand.
Peechanika ChopjittFaculty of Public Health, Kasetsart University Chalermphrakiat Sakon Nakhon Province Campus, Sakon Nakhon, Thailand.ORCID https://orcid.org/0000-0001-5010-4682
Jednipit BorthongDivision of Biology, Faculty of Science and Technology, Rajamangala University of Technology Thanyaburi, Pathumthani, Thailand.
Thidathip WongsurawatSiriraj Long-read Laboratory, Division of Medical Bioinformatics, Department of Research and Development, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
Piroon JenjaroenpunSiriraj Long-read Laboratory, Division of Medical Bioinformatics, Department of Research and Development, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
Rapeephan DuangjanchotSiriraj Long-read Laboratory, Division of Medical Bioinformatics, Department of Research and Development, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
Bhanubong SaiboonjanCenter for Innovation and Standard for Medical Technology and Physical Therapy, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand.
Rujirat HatrongjitFaculty of Science and Engineering, Kasetsart University Chalermphrakiat Sakon Nakhon Province Campus, Sakon Nakhon, Thailand.
Anusak KerdsinFaculty of Public Health, Kasetsart University Chalermphrakiat Sakon Nakhon Province Campus, Sakon Nakhon, Thailand.ORCID https://orcid.org/0000-0003-1055-3656

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Streptococcus suis serotype 14 is the second most prevalent serotype after serotype 2, and is highly prevalent in Southeast Asia. Among the serotype 14 strains, sequence type (ST) 105 is found in humans and pigs. We analysed the genome sequences of S. suis ST105 to identify unique sequences to develop a multiplex PCR (mPCR) -gel electrophoresis and mPCR-lateral flows trip (LFS) for epidemiological purposes. The ST105 genome was closely related to the ST1 genomes. All ST105 of Thai and Vietnamese strains were highly homologous. Of the 1818 genes found in all compared genomes, 36 unique sequences were detected only in the ST105 strain. Of these, two unique sequences encoding hypothetical proteins were selected as PCR targets. Only S. suis ST105 strains were positive for both mPCRs. mPCR-LFS had fewer complications, lower costs, and less time for testing, than those of mPCR-gel electrophoresis. This comparative genomic study demonstrates the usefulness of identifying unique sequences of ST105 S. suis. These unique sequences could be used to develop diagnostic or screening tools, such as PCR, for the detection of specific strains or clones for epidemiological purposes.

Indexed as

Genome, BacterialGenomicsMultiplex Polymerase Chain ReactionStreptococcal InfectionsStreptococcus suisSwine DiseasesAnimalsHumansSwine

Identifiers

PMID40392831
PMCPMC12091807

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.