ArticleVeterinary research communications2025
Immunohistochemistry and molecular biology studies of apelin and apelin receptor in queen placenta.
Article in Veterinary research communications, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Placenta is a tissue where vasculogenesis, blood pressure and blood flow are dramatically important to allow normal embryonic and foetal growth and requires the production of numerous growth factors, hormones and transcription factors. Apelin is a pleiotropic peptide, and its major action relates to energy metabolism, cardiovascular function, body fluid homeostasis via its receptor. The involvement of the apelinergic system during pregnancy in veterinary medicine has been investigated only in bitches. Thereafter, the aim of our study was to investigate, for the first time, presence and distribution of this system in the queen placenta at mid- and end-gestation. Ten pregnant mixed-breed queens were used. The animals were equally divided into two groups based on the stage of pregnancy (mid and end gestation) and, with the written consent of their owners, were subjected to ovariohysterectomy or non-conservative caesarean section. The Real-Time PCR (RT-PCR) analysis showed the presence of transcripts for apelin and its receptor in all the foetal and maternal placenta samples processed. The immunohistochemical (IHC) study evidenced the presence and the distribution of positive immunoreactions for apelin and its receptor in all the samples observed. In particular, in the placental labyrinthic portion, apelin and apelin receptor immunopositivity was evident in the cytoplasm of trophoblasts and endothelial cells. The uterine glands also exhibited a positive immune reaction for apelin and corresponding receptor. Based on our results, apelin and its receptor, also in the queen placenta, could be an important system involved in the physiological development of placenta, embryo and foetal growth.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.