Evidence map›Paper›PMID 40392388›Full record

ArticleMolecular biology reports2025

Comparative analysis of DNA binding and abiotic stress tolerance of Dps All4145 and its homologs in Anabaena PCC 7120.

Shilpi Singh, Krishna Kumar Rai, Alka Shankar, Santosh Kumar Dubey, Alka Raj, Ruchi Rai

Abstract readComparative Study
PubMed Publisher
In one paragraph

Article in Molecular biology reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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1citing papers in PubMed
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1 · What the graph read from it

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2 · The registry

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3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Transcriptional and cellular reprogramming inCurrent research in microbial sciences · 2026
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4 · The record

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5 · Who and what money

Authors and funding

6 authors.

Shilpi SinghMolecular Biology Section, Centre of Advanced Study in Botany, Institute of Science, Banaras Hindu University, Varanasi, 221305, India.
Krishna Kumar RaiMolecular Biology Section, Centre of Advanced Study in Botany, Institute of Science, Banaras Hindu University, Varanasi, 221305, India.
Alka ShankarDepartment of Biosciences, School of Science, Indrashil University, Rajpur, Gujrat, 382740, India.
Santosh Kumar DubeyMolecular Biology Section, Centre of Advanced Study in Botany, Institute of Science, Banaras Hindu University, Varanasi, 221305, India.
Alka RajMolecular Biology Section, Centre of Advanced Study in Botany, Institute of Science, Banaras Hindu University, Varanasi, 221305, India.
Ruchi RaiMolecular Biology Section, Centre of Advanced Study in Botany, Institute of Science, Banaras Hindu University, Varanasi, 221305, India. ruchivrai@yahoo.co.in.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundDNA-binding proteins under starvation (Dps) are mini-ferritins that sequester iron at the ferroxidase center (FOC) of a hollow protein cage. This study characterizes the novel Dps-All4145. Discrepancies between computational predictions and experimental stress responses were addressed using an integrated computational and experimental approach. Additionally, site-directed mutagenesis was performed to explore the mechanistic link between DNA binding and ferroxidation. METHODS AND

resultsThis study involves the molecular characterization of the novel Dps-All4145 and a comparative biochemical and structural analysis of four Dps homologs (Alr3808, All0458, All1173, and All4145) from Anabaena sp. PCC 7120. In-silico and wet lab approaches were employed to assess their biochemical functions, including iron oxidation and DNA protection. The study confirmed that iron oxidation and DNA protection are common attributes of all four Dps homologs. Additionally, all homologs contributed to abiotic stress management. Among them, Alr3808 exhibited the highest efficiency in iron oxidation and DNA protection. Site-directed mutagenesis of Alr3808K49R led to the loss of DNA-binding ability and a 60% reduction in iron oxidation, indicating the crucial role of the N-terminal lysine (K49) residue in these activities.

conclusionThis study provides first-hand insights into the molecular functions of Dps proteins in Anabaena sp. PCC 7120 and highlights the unique significance of K49 in Alr3808 for iron oxidation and DNA binding.

Indexed as

AnabaenaBacterial ProteinsDNA-Binding ProteinsStress, PhysiologicalCeruloplasminIronMutagenesis, Site-DirectedOxidation-ReductionProtein BindingBacterial ProteinsCeruloplasminDNA-Binding ProteinsDPS protein, BacteriaIronDNA bindingDps proteinsOxidative stress response

Identifiers

PMID40392388

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Registered trials

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