ArticleSmall (Weinheim an der Bergstrasse, Germany)2025
Nuclear Assembly in Giant Unilamellar Vesicles Encapsulating Xenopus Egg Extract.
Article in Small (Weinheim an der Bergstrasse, Germany), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
3 citing papers in PubMed.
- Relationship Between Filament-Polymerizing Muscle Myosin and Droplets Generated by Liquid-Liquid Phase Separation.Chembiochem : a European journal of chemical biology · 2026Article
- Lipid composition effects on the number and size of liposomes formed by the inverted emulsion method.Biophysical journal · 2026Article
- Nuclear Assembly in Giant Unilamellar Vesicles Encapsulating Xenopus Egg Extract.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
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Authors and funding
8 authors.
Funding
Abstract
The reconstitution of a cell nucleus in a lipid bilayer-enclosed synthetic cell makes great strides in bottom-up synthetic biology. In this study, a method for assembling a nucleus in giant unilamellar vesicles (GUVs) is proposed. To induce reconstitution of the nucleus, the interphase egg extract of African clawed frogs Xenopus laevis is utilized, known as a biochemically controllable cell-free system capable of transforming an added sperm chromatin into a nucleus in vitro. The GUV formation efficiency is enhanced by the inverted emulsion method through incorporating prolonged waiting time and adding chloroform into lipid-dispersed oil, facilitating subsequent nuclear assembly reactions in the GUVs. Characterization of nucleus-like structures formed in the GUVs revealed the presence of dense DNA and accumulated GFP-NLS in the structure, indicative of functional nuclear import. Immunostaining further validated the presence of nuclear pore complexes on the surfaces of these nucleus-like structures. The approach offers a versatile platform for constructing artificial cellular systems that closely mimic eukaryotic cells.
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Registered trials
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