Evidence map›Paper›PMID 40390491›Full record

ArticleJournal, genetic engineering & biotechnology2025

Assessment of the gold nanoparticles biosynthesized using Casuarina equisetifolia bark extract against the ethion induced Hepato- and neurotoxicity in rats.

Wael Mahmoud Aboulthana, Noha El-Sayed Ibrahim, Amal Gouda Hussien, Amgad Kamal Hassan, Wagdy K B Khalil, Hassan Abdel-Gawad, Hamdy Ahmed Taha, Ayda K Kelany, Kawkab A Ahmed

Abstract read
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In one paragraph

Article in Journal, genetic engineering & biotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Wael Mahmoud AboulthanaBiochemistry Department, Biotechnology Research Institute, National Research Centre, 33 El Bohouth St., P.O. 12622, Dokki, Giza, Egypt. Electronic address: wmkamel83@hotmail.com.
Noha El-Sayed IbrahimMicrobial Biotechnology Department, Biotechnology Research Institute, National Research Centre, 33 El Bohouth St., P.O. 12622, Dokki, Giza, Egypt.
Amal Gouda HussienBiochemistry Department, Biotechnology Research Institute, National Research Centre, 33 El Bohouth St., P.O. 12622, Dokki, Giza, Egypt.
Amgad Kamal HassanBiochemistry Department, Biotechnology Research Institute, National Research Centre, 33 El Bohouth St., P.O. 12622, Dokki, Giza, Egypt.
Wagdy K B KhalilCell Biology Department, Biotechnology Research Institute, National Research Centre, 33 El Bohouth St., P.O. 12622, Dokki, Giza, Egypt.
Hassan Abdel-GawadApplied Organic Chemistry Department, Chemical Industries Researches Institute, National Research Centre, 33 El Bohouth St., P.O. 12622, Dokki, Giza, Egypt.
Hamdy Ahmed TahaApplied Organic Chemistry Department, Chemical Industries Researches Institute, National Research Centre, 33 El Bohouth St., P.O. 12622, Dokki, Giza, Egypt.
Ayda K KelanyDepartment of Genomic Medicine, Cairo University Hospitals, Cairo University, Giza, Cairo, Egypt.
Kawkab A AhmedDepartment of Pathology, Faculty of Veterinary Medicine, Cairo University, Giza 12211, Egypt.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Ethion (Etn) is classified as an organophosphate pesticide (OP) that causes toxicity even at low concentrations and targets the liver, brain, kidney, and blood. Gold nanoparticles (Au-NPs) were biosynthesized from the whole methanolic extract of Casuarina equisetifolia bark, and their efficacy against Etn-induced hepato- and neurotoxicity in rats was assessed. In addition to determining conventional biochemical measurements, the target tissues (liver and brain) were examined for oxidative stress, inflammatory, and fibrotic markers. The protein and isoenzyme patterns were also assayed using an electrophoretic technique. Additionally, apoptotic gene expression was measured. The target tissues were also subjected to histopathological analysis. In all groups treated with C. equisetifolia bark gold nano-extract, it was observed that the levels of the hematological measurements that were impacted by the oral injection of Etn had recovered to normal. Regarding the biochemical measurements, the group that received nano-extract pretreatment showed greater improvement than the therapeutic group. The levels of inflammatory indicators significantly decreased (p ≤ 0.05), while the antioxidant system markers increased in both liver and brain tissues in the group that received the nano-extract beforehand. In both target tissues, especially in the pre-treated group, the nano-extract reduced the severity of the Etn-caused lesions. During electrophoretic assays, the nano-extract in the pre-treated group prevented the qualitative alterations indicated by the lowest similarity index (SI%) values of the Etn-injected group compared to the normal group. The molecular assay showed that the nano-extract reduced the expression of apoptotic genes that were markedly elevated in the Etn-injected rats, but it was unable to return their values to normalcy. The study concluded that in the group that received nano-extract pretreatment, the biochemical, histopathological, physiological, and molecular abnormalities caused by Etn were reduced by the C. equisetifolia bark gold nano-extract.

Indexed as

Casuarina equisetifolia BarkElectrophoresisEthionGene ExpressionGold NanoparticlesIsoenzymes

Identifiers

PMID40390491
PMCPMC12060469

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.